Inicio de sesión

Contact

Dr. Elena Cabezón cabezone@unican.es

Dr. Ignacio Arechaga arechagai@unican.es

Phone: (+34) 942 202033 / Fax : (+34) 942 266399

Mailing address: IBBTEC. C/ Albert Einstein 22, PCTCAN, 39011 Santander 

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Molecular Motors in Nanobiotechnology

grupoElena.jpg


Principal Investigators

Elena Cabezón & Ignacio Arechaga (Assoc. Profs.)


Team

  • Gerardo Carranza Ferrer, postdoctoral
  • Eric Amado, predoctoral
  • Yolanda García Cazorla, predoctoral
  • Fernando Valenzuela, predoctoral

 

Web page

http://grupos.unican.es/Motores_moleculares


Research interests

  • Structural and biochemical approach into the molecular mechanism of RecA/AAA+ motors (ATPases Associated to a large variety of cellular Activities (i.e. DNA unwinding, protein folding, DNA transport…)
  • Molecular motors working on G-quadruplex DNA structures
  •  From DNA transporters to protein transporters: further insights into an evolutionary scenario  
  •  Electron microscopy of large membrane protein complexes


Funding

  • Título del proyecto: "Aplicación de un motor que transloca ssDNA en plataformas de secuenciación de tercera generación"
    Entidad financiadora: Ministerio de Economía y Competitividad
    Referencia: BFU2014-61823-EXP

    Duración: desde 01/09/2015 hasta 31/08/2017

    Investigador responsable: Dr. Elena Cabezón

  • Título del proyecto: "Morfogénesis de membranas bacterianas y mitocondriales: en busca del eslabón perdido"

    Entidad financiadora: Ministerio de Economía y Competitividad

    Referencia : BFU2013_49486-EXP

    Duración: 2014-2016

    Investigador responsable: Dr. Ignacio Arechaga

  • Título del proyecto: "Mecanismos moleculares y evolutivos en motores moleculares que translocan ADN y proteínas"

    Entidad financiadora: Ministerio de Economía y Competitividad

    Referencia: BFU2011-22874

    Duración:desde 01/01/2012 hasta 31/12/2015

    Investigador responsable: Dr. Elena Cabezón


Publications

  • Type IV traffic ATPase TrwD as molecular target to inhibit bacterial conjugation. Ripoll-Rozada J, García-Cazorla Y, Getino M, Machón C, Sanabria-Ríos D, de la Cruz F, Cabezón E, Arechaga I.
    Mol Microbiol
    . 2016 Jun;100(5):912-21. doi: 10.1111/mmi.13359. [pubmed]

  • Towards an integrated model of bacterial conjugation. Cabezón E, Ripoll-Rozada J, Peña A, de la Cruz F, Arechaga I.
    FEMS Microbiol Rev
    . Vol.39:81-95. doi: 10.1111/1574-6976.12085. [pubmed]

  • Molecular motors in bacterial secretion. Peña A, Arechaga I.
    J Mol Microbiol Biotechnol
    . 2013;23(4-5):357-69. doi: 10.1159/000351360. [pubmed]
  • Functional interactions of VirB11 traffic ATPases with VirB4 and VirD4 molecular motors in type IV secretion systems. Ripoll-Rozada J, Zunzunegui S, de la Cruz F, Arechaga I, Cabezón E.
    J Bacteriol
    . 2013 Sep;195(18):4195-201. doi: 10.1128/JB.00437-13. [pubmed]
  • Membrane invaginations in bacteria and mitochondria: common features and evolutionary scenarios. Arechaga I.
    J Mol Microbiol Biotechnol
    . 2013;23(1-2):13-23. doi: 10.1074/jbc.M112.413849. [pubmed]

  • The hexameric structure of a conjugative VirB4 protein ATPase provides new insights for a functional and phylogenetic relationship with DNA translocases. Peña A, Martín-Benito J, Valpuesta JM, Carrascosa JL, de la Cruz F, Cabezón E*, Arechaga I*. J. Biol. Chem. 2012, 287(47), 39925-32. doi: 10.1074/jbc.M112.413849. [pubmed]

  • Ripoll-Rozada J, Peña A, Rivas S, Moro F, de la Cruz F, Cabezón E*,Arechaga I*. Regulation of the type IV secretion ATPase TrwD by magnesium: implications for the catalytic mechanism of the secretion ATPase superfamily. J. Biol. Chem. 2012, 287(21), 17408-14. DOI:10.1074/jbc.M112.357905.
  • Cabezón E*, Lanza VF, Arechaga I*. Membrane-associated nanomotors form macromolecular transport Curr. Opin. Biotechnol. 2012, 23(4), 537-44. DOI:10.1016/j.copbio.2011.11.031.
  • Kumar R, Ferez M, Swamy M, Arechaga I, Rejas MT, Valpuesta JM, Schamel WW, Alarcón B, van Santen HM. Increased sensitivity of antigen-experienced T cells through the enrichment of oligomeric T cell receptor complexes Immunity 2011, 35(3), 375-87. DOI:10.1016/j.immuni.2011.08.010. 
  • Peña A, Ripoll-Rozada J, Zunzunegui S, Cabezón E, de la Cruz F, Arechaga I*. Autoinhibitory regulation of TrwK, an essential VirB4 ATPase in type IV secretion systems J. Biol. Chem. 2011, 286(19), 17376-82. DOI:10.1074/jbc.M110.208942.
  • Canone J, Marino D, Noel LD, Arechaga I, Pichereaux C, Rossignol M, Roby D, Rivas S*. Detection and functional characterization of a 215 amino acid N-terminal extension in the Xanthomonas type III effector XopD PLOS One 2010, 22(5), e15773. DOI:10.1371/journal.pone.0015773.
  •  Arechaga I, Swamy M, Abia D, Schamel WW, Alarcón B, Valpuesta JM*. Structural characterization of the TCR complex by electron microscopy Int. Immunol. 2010, 22(11), 897-903. DOI:10.1093/intimm/dxq443.
  • Matilla I, Alfonso C, Rivas G, Bolt EL, de la Cruz F, Cabezón E*. The conjugative DNA translocase TrwB is a structure specific DNA-binding protein. J. Biol. Chem. 2010, 285(23, 17537-44. DOI:10.1074/jbc.M109.084137.
  •  Arechaga I, Martínez-Costa OH, Ferreras C, Carrascosa JL, Aragón JJ*. Electron microscopy analysis of mammalian phosphofructokinase reveals an unusual 3-dimensional structure with significant implications for enzyme function FASEB J. 2010, 24(12), 4960-8. DOI:10.0024-4960.
  • Mihajlovic S, Lang S, Sut MW, Strohmaier H, Gruber CJ, Koraimann G, Cabezón E, Moncalián G, de la Cruz F,Zechner EL* . Plasmid r1 conjugative DNA processing is regulated at the coupling protein interface. J. Bacteriol. 2009, 19(22), 6877-87. DOI:10.1128/JB.00918-09.
  • Arechaga I*, Peña A, Zunzunegui S, Del Carmen Fernández-Alonso M, Rivas G, de la Cruz F. ATPase activity and oligomeric state of TrwK, the VirB4 homologue of plasmid R388 Type IV secretion system. J. Bacteriol. 2008, 190(15), 5472-9. DOI:10.1128/JB.00321-08.
  • Tato I, Matilla I, Arechaga I, Zunzunegui S, de la Cruz F, Cabezón E*. The ATPase activity of the DNA transporter TrwB is modulated by protein TrwA: Implications for a common assembly mechanism of DNA translocating motors. J. Biol Chem. 2007, 282(35), 25569-76. DOI:10.1074/jbc.M703464200.
  • Arechaga I*, Fotiadis D. Reconstitution of mitochondrial ATP synthase into lipid bilayers for structural analysis. J. Struct. Biol. 160(3, 287-94. DOI:10.1016/j.jsb.2007.09.07.
  • Cabezón E, de la Cruz F "TrwB: An F1-ATPase-like molecular motor involved in DNA transport during bacterial conjugation". Res. Microbiol. 2006, 157:299–305. DOI:10.1016/j.resmic.2005.12.002.
  • Schamel W W A, Arechaga I, Risueño RM, van Santen HM, Cabezas P, Risco C, Valpuesta JM, Alarcón B. "Coexistence of multivalent and monovalent TCRs explains high sensitivity and wide range of response". J Exp Med. 2005, 202:493–503. DOI:10.1084/jem.20042155.
  • Tato I, Zunzunegui S, de la Cruz F, Cabezón E*. "TrwB, the coupling protein involved in DNA transport during bacterial conjugation, is a DNA-dependent ATPase". Proc Natl Acad Sci USA 2005, 102:8156-61. DOI:10.1073/pnas.0503402102.
  • Cabezón E, Montgomery MG, Leslie AG, Walker JE*. (2003) "The structure of bovine F1-ATPase in complex with its regulatory protein IF1." Nat Struct Biol. 10(9):744-50. DOI:10.1038/nsb966.
  • Arechaga, I., Miroux, B., Runswick, M. J. & Walker, J. E*. (2003). "Over-expression of Escherichia coli F1F(o)-ATPase subunit a is inhibited by instability of the uncB gene transcript". FEBS Lett 547, 97-100. DOI:10.1016/S0014-5793(03)00677-X. 
  • Jekabsons, M. B., Echtay, K. S., Arechaga, I. & Brand, M. D. (2003). "Molecular properties of purified human uncoupling protein 2 refolded from bacterial inclusion bodies". J Bioenerg Biomembr 35, 409-18. DOI:10.1023/A:1027335713635.
  • Murata, T*., Arechaga, I*., Fearnley, I. M., Kakinuma, Y., Yamato, I. & Walker, J. E*. (2003). (*equal contributors) "The membrane domain of the Na+-motive V-ATPase from Enterococcus hirae contains a heptameric rotor". J Biol Chem 278, 21162-7. DOI:10.1074/jbc.M301620200
  • Martinez LO, Jacquet S, Esteve JP, Rolland C, Cabezon E, Champagne E, Pineau T, Georgeaud V, Walker JE, Terce F, Collet X, Perret B, Barbaras R*. (2003) "Ectopic beta-chain of ATP synthase is an apolipoprotein A-I receptor in hepatic HDL endocytosis". Nature 421:75-9. DOI:10.1038/nature01250.