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a3B4 Acetylcholine Nicotinic Receptors Are Components of theSecretory Machinery Clusters in Chromaffin Cells

Abstract: The heteromeric assembly of a3 and B4 subunits of acetylcholine nicotinic receptors (nAChRs) seems to mediate the secretory response in bovine chromaffin cells. However, there is no information about the localization of these nAChRs in relationship with the secretory active zones in this cellular model. The present work presents the first evidence that, in fact, a population of these receptors is associated through the F-actin cytoskeleton with exocytotic machinery components, as detected by SNAP-25 labeling. Furthermore, we also prove that, upon stimulation, the probability to find ?3?4 nAChRs very close to exocytotic events increases with randomized distributions, thus substantiating the clear dynamic behavior of these receptors during the secretory process. Modeling on secretory dynamics and secretory component distributions supports the idea that a3B4 nAChR cluster mobility could help with improving the efficiency of the secretory response of chromaffin cells. Our study is limited by the use of conventional confocal microscopy; in this sense, a strengthening to our conclusions could come from the use of super-resolution microscopy techniques in the near future

 Fuente: International journal of molecular sciences, 2022, 23(16), 9101

 Editorial: MDPI

 Fecha de publicación: 14/08/2022

 Nº de páginas: 13

 Tipo de publicación: Artículo de Revista

 DOI: 10.3390/ijms23169101

 ISSN: 1661-6596,1422-0067

 Proyecto español: PID2020-114824GB-I00

Autoría

VILLANUEVA, JOSÉ

MANUEL CRIADO HERRERO

GIMÉNEZ-MOLINA, YOLANDA

VIRGINIA GONZALEZ VELEZ

LUIS MIGUEL GUTIERREZ PEREZ