Buscar

Estamos realizando la búsqueda. Por favor, espere...

Incorporation of Tb and Gd improves the diagnostic functionality of magnetotactic bacteria

Abstract: Magnetotactic bacteria are envisaged as potential theranostic agents. Their internal magnetic compass, chemical environment specificity and natural motility enable these microorganisms to behave as nanorobots, as they can be tracked and guided towards specific regions in the body and activated to generate a therapeutic response. Here we provide additional diagnostic functionalities to magnetotactic bacteria Magnetospirillum gryphiswaldense MSR-1 while retaining their intrinsic capabilities. These additional functionalities are achieved by incorporating Tb or Gd in the bacteria by culturing them in Tb/Gd supplemented media. The incorporation of Tb provides luminescence properties, enabling potential applications of bacteria as biomarkers. The incorporation of Gd turns bacteria into dual contrast agents for magnetic resonance imaging, since Gd adds T1 contrast to the existing T2 contrast of unmodified bacteria. Given their potential clinical applications, the diagnostic ability of the modified MSR-1 has been successfully tested in vitro in two cell models, confirming their suitability as fluorescent markers (Tb-MSR-1) and dual contrast agents for MRI (Gd-MSR-1).

 Autoría: Gandarias L., Jefremovas E.M., Gandia D., Marcano L., Martínez-Martínez V., Ramos-Cabrer P., Chevrier D.M., Valencia S., Fernández Barquín L., Fdez-Gubieda M.L., Alonso J., García-Prieto A., Muela A.,

 Fuente: Materials Today Bio, 2023, 20, 100680

 Editorial: Elsevier

 Fecha de publicación: 01/06/2023

 Nº de páginas: 11

 Tipo de publicación: Artículo de Revista

 DOI: 10.1016/j.mtbio.2023.100680

 ISSN: 2590-0064

 Proyecto español: PID2020-115704RB-C31

 Url de la publicación: https://doi.org/10.1016/j.mtbio.2023.100680

Autoría

GANDARIAS, LUCÍA

ELIZABETH MARTIN JEFREMOVAS

GANDIA, DAVID

MARCANO, LOURDES

MARTÍNEZ-MARTÍNEZ, VIRGINIA

RAMOS-CABRER, PEDRO

CHEVRIER, DANIEL M.

VALENCIA, SERGIO

FDEZ-GUBIEDA, M. LUISA

GARCÍA-PRIETO, ANA

MUELA, ALICIA