Inicio de sesión

​​Contact

Dr. Fernando de la Cruz


Phone: (+34) 942 201942 / Fax : (+34) 942 266399

Email: delacruz@unican.es

Mailing address: IBBTEC. C/ Albert Einstein 22, PCTCAN, 39011 Santander

Intergenomics

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Principal investigator

Dr. Fernando de la Cruz, Full Professor


Other personnel

  • Raúl Fernández López, postdoctoral
  • M. Pilar Garcillán Barcia, postdoctoral
  • Marta Robledo Garrido, postdoctoral
  • Miguel Baez, predoctoral
  • Roger Bartomeus, predoctoral
  • Alfonso Mendaña, predoctoral
  • Carolina Palencia Gándara, predoctoral
  • Jorge Rodríguez, predoctoral
  • Irene Sanz, predoctoral
  • Sheila González Gutiérrez, laboratory technician
  • Santiago Redondo Salvo, laboratory technician


Web page

 http://grupos.unican.es/intergenomica/index.htm


Research lines

– Evolution and Ecology:
         • Classification of conjugative and mobilizable plasmids based on their relaxase sequences.
         • Conjugative systems of Cyanobacteria and Actinomycetes. 

– Molecular Engines: Protein and DNA transporters across bacterial membranes

– Protein Engineering: Conjugative relaxases as a target for the inhibition of the dissemination of antibiotic resistance.

– Systems Biology


Funding


- Nuevas estrategias para el control de infecciones nosocomiales. INNOECO RTC-2015-3184-1.

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​ ​“Promover el desarrollo tecnológico, la innovación y una investigación de calidad”


- Plásmidos ortogonales para Biología Sintética y de Sistemas. Ministerio de Educación (BFU2011-26608).
- Bacterial Computing with Engineered Populations (BACTOCOM). European Union 7th Framework. Programme, Area Information and Communication Technologies (FP7-ICT-2009-4; Proyecto nº 248919).
- EVOTAR: Evolution and transfer of antibiotic resistance (Project no. 282004). European Union FP7-HEALTH 2011.2.3.1-2.
- Diversidad y distribución de plásmidos de bacterias ácido-lácticas. Acción Integrada Hispano-Serbia. PRI-AIBSE-2011-1186. IP: Fernando de la Cruz

- INGENIERÍA GENÉTICA DE ESCHERICHIA COLI PARA MEJORAR LA PRODUCCIÓN DE LÍPIDOS RICOS EN ÁCIDOS GRASOS OMEGA-3 (TF16-XX-003), cofinanciado por SODERCAN S.A. y el Programa Operativo FEDER


Last Publications


  • Genomics of high molecular weight plasmids isolated from an on-farm biopurification system. Martini MC, Wibberg D, Lozano M, Torres Tejerizo G, Albicoro FJ, Jaenicke S, van Elsas JD, Petroni A, Garcillán-Barcia MP, de la Cruz F, Schlüter A, Pühler A, Pistorio M, Lagares A, Del Papa MF.
    Sci Rep
    . 2016 Jun 20;6:28284. doi: 10.1038/srep28284. [PubMed]

  • Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells. Okumus B, Landgraf D, Lai GC, Bakhsi S, Arias-Castro JC, Yildiz S, Huh D, Fernandez-Lopez R, Peterson CN, Toprak E, El Karoui M, Paulsson J.
    Nat Commun
    . 2016 May 18;7:11641. doi: 10.1038/ncomms11641. [PubMed]

  • Genomics of high molecular weight plasmids isolated from an on-farm biopurification system. Martini MC, Wibberg D, Lozano M, Torres Tejerizo G, Albicoro FJ, Jaenicke S, van Elsas JD, Petroni A, Garcillán-Barcia MP, de la Cruz F, Schlüter A, Pühler A, Pistorio M, Lagares A, Del Papa MF.
    Sci Rep
    . 2016 Jun 20;6:28284. doi: 10.1038/srep28284. [PubMed]

  • Concerted action of NIC relaxase and auxiliary protein MobC in RA3 plasmid conjugation. Godziszewska J, Moncalián G, Cabezas M, Bartosik AA, de la Cruz F, Jagura-Burdzy G.
    Mol Microbiol
    . 2016 Aug;101(3):439-56. doi: 10.1111/mmi.13401. Epub 2016 Jun 2. [PubMed]

  • Type IV traffic ATPase TrwD as molecular target to inhibit bacterial conjugation. Ripoll-Rozada J, García-Cazorla Y, Getino M, Machón C, Sanabria-Ríos D, de la Cruz F, Cabezón E, Arechaga I.
    Mol Microbiol. 2016 Jun;100(5):912-21. doi: 10.1111/mmi.13359. Epub 2016 Mar 22. [PubMed]

  • Rebooting the genome: The role of negative feedback in horizontal gene transfer. Fernandez-Lopez R, de la Cruz F.

    Mob Genet Elements. 2015 Feb 23;4(6):1-6. eCollection 2014 Nov-Dec. [PubMed]

  • Towards an integrated model of bacterial conjugation. Cabezón E, Ripoll-Rozada J, Peña A, de la Cruz F, Arechaga I.
    FEMS Microbiol Rev. 2015 Jan;39(1):81-95. doi: 10.1111/1574-6976.12085. Epub 2014 Dec 4. Review. [pubmed]

  • Degenerate primer MOB typing of multiresistant clinical isolates of E. coli uncovers new plasmid backbones. Garcillán-Barcia MP, Ruiz Del Castillo B, Alvarado A, de la Cruz F, Martínez-Martínez L.
    Plasmid
    . 2015 Jan;77:17-27. doi: 10.1016/j.plasmid.2014.11.003. [pubmed]

  • Dissemination of Cephalosporin Resistance Genes between Escherichia coli Strains from Farm Animals and Humans by Specific Plasmid Lineages. de Been M, Lanza VF, de Toro M, Scharringa J, Dohmen W, Du Y, Hu J, Lei Y, Li N, Tooming-Klunderud A, Heederik DJ, Fluit AC, Bonten MJ, Willems RJ, de la Cruz F, van Schaik W.

    PLoS Genet. 2014 Dec 18;10(12):e1004776. doi: 10.1371/journal.pgen.1004776. [pubmed]

  • Plasmid Flux in Escherichia coli ST131 Sublineages, Analyzed by Plasmid Constellation Network (PLACNET), a New Method for Plasmid Reconstruction from Whole Genome Sequences. Lanza VF, de Toro M, Garcillán-Barcia MP, Mora A, Blanco J, Coque TM, de la Cruz F.

    PLoS Genet. 2014 Dec 18;10(12):e1004766. doi: 10.1371/journal.pgen.1004766. [pubmed]

  • Molecular epidemiology and virulence of Escherichia coli O16:H5-ST131: comparison with H30 and H30-Rx subclones of O25b:H4-ST131. Dahbi G, Mora A, Mamani R, López C, Alonso MP, Marzoa J, Blanco M, Herrera A, Viso S, García-Garrote F, Tchesnokova V, Billig M, de la Cruz F, de Toro M, González-López JJ, Prats G, Chaves F, Martínez-Martínez L, López-Cerezo L, Denamur E, Blanco J.
    Int J Med Microbiol
    . 2014 Nov;304(8):1247-57. doi: 10.1016/j.ijmm.2014.10.002. [pubmed]

  • A high security double lock and key mechanism in HUH relaxases controls oriT-processing for plasmid conjugation. Carballeira JD, González-Pérez B, Moncalián G, de la Cruz F.
    Nucleic Acids Res
    . 2014;42(16):10632-43. doi: 10.1093/nar/gku741.
    [pubmed]
  • Bringing them together: plasmid pMV158 rolling circle replication and conjugation under an evolutionary perspective.Lorenzo-Díaz F, Fernández-López C, Garcillán-Barcia MP, Espinosa M.Plasmid. 2014 Jul;74:15-31. doi: 10.1016/j.plasmid.2014.05.004. [pubmed]
  • Use of limited proteolysis and mutagenesis to identify folding domains and sequence motifs critical for wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase activity. Villa JA, Cabezas M, de la Cruz F, Moncalián G.
    Appl Environ Microbiol
    . 2014 Feb;80(3):1132-41. doi: 10.1128/AEM.03433-13. [pubmed]
  • PipX, the coactivator of NtcA, is a global regulator in cyanobacteria. Espinosa J, Rodríguez-Mateos F, Salinas P, Lanza VF, Dixon R, de la Cruz F, Contreras A.
    Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):E2423-30. doi: 10.1073/pnas.1404097111. [pubmed]
  • Key components of the eight classes of type IV secretion systems involved in bacterial conjugation or protein secretion. Guglielmini J, Néron B, Abby SS, Garcillán-Barcia MP, de la Cruz F, Rocha EP.
    Nucleic Acids Res. 2014;42(9):5715-27. doi: 10.1093/nar/gku194. [pubmed]
  • Negative feedback and transcriptional overshooting in a regulatory network for horizontal gene transfer. Fernandez-Lopez R, Del Campo I, Revilla C, Cuevas A, de la Cruz F.
    PLoS Genet. 2014 Feb 27;10(2):e1004171. doi: 10.1371/journal.pgen.1004171. [pubmed]
  • Plasmid conjugation from proteobacteria as evidence for the origin of xenologous genes in cyanobacteria. Encinas D, Garcillán-Barcia MP, Santos-Merino M, Delaye L, Moya A, de la Cruz F.
    J Bacteriol. 2014 Apr;196(8):1551-9. doi: 10.1128/JB.01464-13. [pubmed]
  • Use of limited proteolysis and mutagenesis to identify folding domains and sequence motifs critical for wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase activity. Villa JA, Cabezas M, de la Cruz F, Moncalián G.
    Appl Environ Microbiol. 2014 Feb;80(3):1132-41. doi: 10.1128/AEM.03433-13. [pubmed]
  • Genomic analysis of the emergence and evolution of multidrug resistance during a Klebsiella pneumoniae outbreak including carbapenem and colistin resistance. López-Camacho E, Gómez-Gil R, Tobes R, Manrique M, Lorenzo M, Galván B, Salvarelli E, Moatassim Y, Salanueva IJ, Pareja E, Codoñer FM, Alvarez-Tejado M, Garcillán-Barcia MP, De la Cruz F, Mingorance J.
    J Antimicrob Chemother. 2013 Oct 23. [pubmed]
  • Subcellular location of the coupling protein TrwB and the role of its transmembrane domain. Segura RL, Aguila-Arcos S, Ugarte-Uribe B, Vecino AJ, de la Cruz F, Goñi FM, Alkorta I.
    Biochim Biophys Acta. 2013 Sep 6;1838(1PB):223-230. doi: 10.1016/j.bbamem.2013.08.016. [pubmed]
  • Four main virotypes among extended-spectrum-β-lactamase-producing isolates of Escherichia coli O25b:H4-B2-ST131: bacterial, epidemiological, and clinical characteristics. Blanco J, Mora A, Mamani R, López C, Blanco M, Dahbi G, Herrera A, Marzoa J, Fernández V, de la Cruz F, Martínez-Martínez L, Alonso MP, Nicolas-Chanoine MH, Johnson JR, Johnston B, López-Cerero L, Pascual A, Rodríguez-Baño J; Spanish Group for Nosocomial Infections (GEIH).
    J Clin Microbiol. 2013 Oct;51(10):3358-67. doi: 10.1128/JCM.01555-13. [pubmed]
  • Catalytic domain of plasmid pAD1 relaxase TraX defines a group of relaxases related to restriction endonucleases. Francia MV, Clewell DB, de la Cruz F, Moncalián G.
    Proc Natl Acad Sci U S A. 2013 Aug 13;110(33):13606-11. doi: 10.1073/pnas.1310037110. Epub 2013 Jul 31. [pubmed]
  • Functional interactions of VirB11 traffic ATPases with VirB4 and VirD4 molecular motors in type IV secretion systems. Ripoll-Rozada J, Zunzunegui S, de la Cruz F, Arechaga I, Cabezón E.
    J Bacteriol. 2013 Sep;195(18):4195-201. doi: 10.1128/JB.00437-13. [pubmed]
  • Multicellular computing using conjugation for wiring. Goñi-Moreno A, Amos M, de la Cruz F.
    PLoS One. 2013 Jun 20;8(6):e65986. doi: 10.1371/journal.pone.0065986. [pubmed]
  • Breaking and joining single-stranded DNA: the HUH endonuclease superfamily. Chandler M, de la Cruz F, Dyda F, Hickman AB, Moncalian G, Ton-Hoang B.
    Nat Rev Microbiol. 2013 Aug;11(8):525-38. doi: 10.1038/nrmicro3067. [pubmed]
  • The transmembrane domain of the T4SS coupling protein TrwB and its role in protein-protein interactions. Segura RL, Aguila-Arcos S, Ugarte-Uribe B, Vecino AJ, de la Cruz F, Goñi FM, Alkorta I.
    Biochim Biophys Acta. 2013 Sep;1828(9):2015-25. doi: 10.1016/j.bbamem.2013.05.022. [pubmed]
  • Ordering the bestiary of genetic elements transmissible by conjugation. Garcillán-Barcia MP, de la Cruz F.
    Mob Genet Elements. 2013 Jan 1;3(1):e24263. [pubmed]
  • Structural independence of conjugative coupling protein TrwB from its Type IV secretion machinery. Larrea D, de Paz HD, Arechaga I, de la Cruz F, Llosa M.
    Plasmid. 2013 Jul;70(1):146-53. doi: 10.1016/j.plasmid.2013.03.006. [pubmed]
  • CRISPR-Cas systems preferentially target the leading regions of MOBF conjugative plasmids. Westra ER, Staals RH, Gort G, Høgh S, Neumann S, de la Cruz F, Fineran PC, Brouns SJ.
    RNA Biol. 2013 May;10(5):749-61. doi: 10.4161/rna.24202. [pubmed]
  • Evolution of Conjugation and Type IV Secretion Systems. Guglielmini J, de la Cruz F, Rocha EP.
    Mol Biol Evol. 2013 Feb;30(2):315-31. doi: 10.1093/molbev/mss221. [pubmed]
  • A degenerate primer MOB typing (DPMT) method to classify gamma-proteobacterial plasmids in clinical and environmental settings. Alvarado A, Garcillán-Barcia MP, de la Cruz F.
    PLoS One. 2012;7(7):e40438. [pubmed]
  • Determination of conjugation rates on solid surfaces. Del Campo I, Ruiz R, Cuevas A, Revilla C, Vielva L, de la Cruz F.
    Plasmid. 2012 Mar;67(2):174-82. [pubmed]
  • Interaction between relaxase MbeA and accessory protein MbeC of the conjugally mobilizable plasmid ColE1. Varsaki A, Lamb HK, Eleftheriadou O, Vandera E, Thompson P, Moncalián G, de la Cruz F, Hawkins AR, Drainas C.
    FEBS Lett. 2012 Mar 23;586(6):675-9. [pubmed]