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​​Contact

Dr. Matxalen Llosa


Phone: (+34) 942 201957 / Fax : (+34) 942 266399
Email: llosam@unican.es
Mailing address: IBBTEC. C/ Albert Einstein 22, PCTCAN, 39011 Santande​r

Biology of Bacterial Type IV Secretion Systems




Principal Investigator

Dr. Matxalen Llosa, Full Professor


Other personnel

  • Pablo Guridi, predoctoral
  • Dolores Lucía Guzmán Herrador, predoctoral
  • Sara Samperio, predoctoral
  • Rigoberto May Cuz, Erasmus student


Lab Retreat 2019


grupMtxRt19.jpg


 


 

Research lines

  •  Use of bacterial secretion systems for the genomic modification of human cells
  • Site-specific recombination and integration catalyzed by conjugative relaxases 
  • Substrate Recruitment by Type IV Secretion systems

 

Funding:

  • MINECO BIO2017-87190-R, “Secreción de ADN de bacterias a células de mamífero. Análisis de su papel en virulencia y su potencial aplicación terapéutica”. IP: Matxalen Llosa

  • AECC IDEAS211LLOS, "Papel de la secreción de ADN bacteriano en el desarrollo tumoral". IP: Matxalen Llosa
  • SODERCAN/FEDER JU06. “Reclutamiento de Proteínas y ADN por sistemas de secreción Tipo IV A y B”. IP: Matxalen Llosa


Relevant publications (last 10 years)

  • The secret life of conjugative relaxases.
    Guzmán-Herrador DL, Llosa M.

    Plasmid. 2019, 104:102415. doi: 10.1016/j.plasmid.2019.102415. [PubMed]

  • Exchange of functional domains between a bacterial conjugative relaxase and the integrase of the human adeno-associated virus. 

    Agúndez L, Zárate-Pérez F, Meier AF, Bardelli M, Llosa M, Escalante CR, Linden RM, Henckaerts E.
    PLoS ONE 2018  13 (7): e0200841. https://doi.org/10.1371/journal. pone.0200841. [pubmed]

  • Coupling proteins in Type IV Secretion. Llosa M, Alkorta I. In: S Backert & E Grohmann (Eds). Type IV secretion in Gram-negative and Gram-positive bacteria”. 
    Curr Topics Microbiol Immunol 2017 
    413:143-168. Doi: 10.1007/978-3-319-75241-9_6. [pubmed]

  • DNA delivery and genomic integration into mammalian target cells through Type IV A and B secretion systems of human pathogens. Guzmán-Herrador DL, Steiner S, Alperi A, González-Prieto C, Roy CR, Llosa M.
    Front Microbiol. 2017 Aug 22; 8:1503. doi: 10.3389/fmicb.2017.01503 [pubmed]

  • Substrate translocation involves specific lysine residues of the central channel of the conjugative coupling protein TrwB. Larrea D, de Paz HD, Matilla I, Guzmán-Herrador DL, Lasso G, de la Cruz F, Cabezón E, Llosa M.
    Mol Genet Genomics. 2017, 292:1037-1049. doi: 10.1007/s00438-017-1331-3 [pubmed]
  • The Conjugative Relaxase TrwC Promotes Integration of Foreign DNA in the Human Genome. González-Prieto C, Gabriel R, Dehio C, Schmidt M, Llosa M. 
    Appl Environ Microbiol. 2017 83:e00207-17. doi: 10.1128/AEM.00207-17. [Epub ahead of print] [pubmed]
  • A Functional oriT in the Ptw Plasmid of Burkholderia cenocepacia Can Be Recognized by the R388 Relaxase TrwC. Fernández-González E, Bakioui S, Gomes MC, O'Callaghan D, Vergunst AC, Sangari FJ, Llosa M.
    Front Mol Biosci
    . 2016 May 3;3:16. doi: 10.3389/fmolb.2016.00016. eCollection 2016. [pubmed]

  • Chloramphenicol Selection of IS10 Transposition in the cat Promoter Region of Widely Used Cloning Vectors. González-Prieto C, Agúndez L, Llosa M.
    PLoS One
    . 2015 Sep 16;10(9):e0138615. doi: 10.1371/journal.pone.0138615. eCollection 2015. [pubmed]
  • A translocation motif in relaxase TrwC specifically affects recruitment by its conjugative type IV secretion system. Alperi A, Larrea D, Fernández-González E, Dehio C, Zechner EL, Llosa M.
    J Bacteriol
    . 2013 Nov;195(22):4999-5006. doi: 10.1128/JB.00367-13. Epub 2013 Aug 30. [pubmed]
  • Structural independence of conjugative coupling protein TrwB from its Type IV secretion machinery. Larrea D, de Paz HD, Arechaga I, de la Cruz F, Llosa M.
    Plasmid
    . 2013 Jul;70(1):146-53. doi: 10.1016/j.plasmid.2013.03.006. Epub 2013 Apr 10. [pubmed]

  • HUH site-specific recombinases for targeted modification of the human genome. González-Prieto C, Agúndez L, Linden RM, Llosa M.
    Trends Biotechnol
    . 2013 May;31(5):305-12. doi: 10.1016/j.tibtech.2013.02.002. Epub 2013 Mar 30. Review. [pubmed]

  • Site-specific integration of foreign DNA into minimal bacterial and human target sequences mediated by a conjugative relaxase. Agúndez L, González-Prieto C, Machón C, Llosa M.
    PLoS One
    . 2012;7(1):e31047. doi: 10.1371/journal.pone.0031047. Epub 2012 Jan 23. [pubmed]

  • New perspectives into bacterial DNA transfer to human cells. Llosa M, Schröder G, Dehio C.
    Trends Microbiol
    . 2012 Aug;20(8):355-9. doi: 10.1016/j.tim.2012.05.008. Epub 2012 Jun 29. Review. [pubmed]

  • Fernández-González E, de Paz HD, Alperi A, Agúndez L, Faustmann M, Sangari FJ, Dehio C, Llosa M (2011). "Transfer of R388 derivatives by a pathogenesis-associated type IV secretion system into both bacteria and human cells". J Bacteriol 193 (22), 6257–6265
  • Agúndez L, Machón C, César CE, Rosa-Garrido M, Delgado MD, Llosa M (2011). "Nuclear targeting of a bacterial integrase which mediates site-specific recombination between bacterial and human target sequences". Appl Env Microbiol 77(1): 201-210
  • de Paz HD, Larrea D, Zunzunegui S, Dehio C, de la Cruz F, Llosa M (2010). "Functional dissection of the conjugative coupling protein TrwB". J Bacteriol 192(11), 2655-2669.
  • Llosa M, Roy C, Dehio C (2009). Bacterial Type IV secretion systems in human disease. Mol. Microbiol. 73(2):141-151.
  • César CE, Llosa M (2007). TrwC-mediated site-specific recombination is controlled by host factors altering local DNA topology. J. Bacteriol. 189(24):9037-9043.
  • César CE, Machón C, de la Cruz F, Llosa M (2006). A new domain of conjugative relaxase TrwC responsible for efficient oriT-specific recombination on minimal target sequences. Mol. Microbiol. 62(4):984-996.
  • Draper O, César CE, Machón C, de la Cruz F, Llosa M (2005). Site-specific recombinase and integrase activities of a conjugative relaxase in the recipient cell. Proc. Natl. Acad. Sci. USA 102:16385-16390.
  • de Paz HD, Sangari FJ, Bolland S, García-Lobo JM, Dehio C, de la Cruz F, Llosa M (2005). Functional interactions between Type IV secretion systems involved in DNA transfer and virulence. Microbiology 151:3505-3516.
  • Llosa M, de la Cruz F (2005). Bacterial conjugation: a potential tool for genomic engineering. Res. Microbiol. 156:1-6.
  • Llosa M, O´Callaghan D (2004) Euroconference on the Biology of Type IV Secretion Processes: Bacterial gates into the outer world. Mol. Microbiol. 53:1-8.
  • Llosa M, Zunzunegui S, de la Cruz F (2003). Conjugative coupling proteins interact with cognate and heterologous VirB10-like proteins while exhibiting specificity for cognate relaxosomes. Proc. Natl. Acad. Sci. USA 100:10460-10464.
  • Llosa M, Gomis-Rüth X, Coll M, de la Cruz F (2002). Bacterial conjugation. A two-step model for DNA transport. Mol. Microbiol. 45:1-8.