Gabriel Moncalián Montes (IP)

  • IBBTEC. Albert Einstein, 22; 39011 Santander
  • gabriel.moncalian@unican.es
  • 942 201934
  • Protein Design and Engineering
  • Principal Investigator
  • Structural Biology
  • Department of Microbiology & Genomics

Predoc (1994-2000): BSc in Chemical Sciences by the University of Valladolid. Doctoral thesis on the nucleoprotein complex that initiates bacterial conjugation supervised by Dr. Fernando de la Cruz in the Department of Molecular Biology of the University of Cantabria thanks to a MEC fellowship, with short term stays in the laboratories of Dr. Robert T. Sauer at MIT (Cambridge, MA) and Dr. Ellen Zechner at theUniversity of Graz (Austria). Extraordinary doctorate award.

Postdoc 1 (2000-2003): Supervised by Dr. John A. Tainer at the Scripps Research Institute (La Jolla, CA) working on structural biology of DNA Repair.

Postdoc 2 (2003-2004): Supervised by Dr. Jerónimo Bravo at the CNIO (Madrid) working on structural biology of endocytosis.

Ramón y Cajal Contract (2005-2009): Reincorporation to the UC. Start of an independent project on the synthesis of triglycerides and polyunsaturated fatty acids.

Present: Associate Professor of Genetics at the University of Cantabria since 2009 and Principal Investigator of the Protein Design and Engineering Research Group at the Institute of Biomedicine and Biotechnology of Cantabria (IBBTEC) since 2014. In this last stage I have consolidated my research group. PI of different public national research projects and projects with national companies, highlighting the contract with Sniace to develop a zenith-E project for the improvement of bioethanol production. Regular user of the ALBA synchrotron. Coordinator of the master's and doctoral programs in Molecular Biology and Biomedicine and vice-dean of the Faculty of Medicine. I have published 40 articles in international journals (Nature, Cell, NSMB, PNAS, EMBOJ, Angewandte Chemie, etc), a patent for the production of triglycerides in bacteria and I have supervised eight doctoral theses.

​Protein Design and Engineering


Research Lines

    • Structure, function and modification of polyunsaturated fatty acid synthases.
    • Optimization of the microbial production of added value fatty acids and triglycerides.
    • Optimization of proteins involved in bacterial conjugation for biotechnological applications. 

Services offered 

    • Design and optimization of proteins with biotechnological, biomedical or industrial interest. 
    • Structural analysis of proteins by X-ray crystallography and in silico molecular threading.

Funding 

    • Plasmid Offensive: Protein engineering operations (BFU2014-55534-C2-2-P)
    • Probióticos guardianes para la lucha contra la resistencia a antibióticos mediante sistemas CRISPR conjugativos (BFU2014-62007-EXP)
    • Optimización de la purificación de la catalasa obtenida de hígado de vaca (TF16-XX-007 –Laboratorios Arroyo S.L.- SODERCAN/FEDER)
    • Desarrollo de tecnología amti para el cultivo de algas en plantas de acuicultura convencionales. La biotecnología como instrumento para una acuicultura rentable y circular (Investalga S.L.).

Reprogramming microorganisms for the biosynthesis of astaxanthin via metabolic engineering

Wan, X.; Zhou, X-R.; Moncalian, G.; Su, L.; Chen, W-C.; Zhu, H-Z.; Chen, D.; Gong, Y-M.; Huang, F-H. and Deng, Q-C.

Prog Lipid Res. 2021 Jan;81:101083. doi: 10.1016/j.plipres.2020.101083. Epub 2020 Dec 26.


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Structure and Mechanism of the Ketosynthase-Chain Length Factor Didomain from a Prototypical Polyunsaturated Fatty Acid Synthase

Santín, O., Yuet, K., Khosla, C. and Moncalián, G.

​Biochemistry. 2020 Dec 22;59(50):4735-4743. doi: 10.1021/acs.biochem.0c00785. Epub 2020 Dec 7.


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Integrated strategy for the separation of endotoxins from biofluids. LPS capture on newly synthesized protein

Integrated strategy for the separation of endotoxins from biofluids. LPS capture on newly synthesized protein

Basauri A., Fallanza M., Giner-Robles L., Fernandez-Lopez R., Moncalián G., de la Cruz F., Ortiz I.

Separation and Purification Technology. Volume 255, 15 January 2021, 117689



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Biochemical interactions between LPS and LPS-binding molecules

Basauri A, González-Fernández C, Fallanza M, Bringas E, Fernandez-Lopez R, Giner L, Moncalián G, de la Cruz F, Ortiz I.

​Crit Rev Biotechnol. 2020 May;40(3):292-305. doi: 10.1080/07388551.2019.1709797. Epub 2020 Jan 13.

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ArdC, a ssDNA-binding protein with a metalloprotease domain, overpasses the recipient hsdRMS restriction system broadening conjugation host range

Lorena González-Montes, Irene Del Campo, M Pilar Garcillán-Barcia, Fernando de la Cruz, Gabriel Moncalián

​PLoS Genet. 2020 Apr 29;16(4):e1008750. doi: 10.1371/journal.pgen.1008750. eCollection 2020 Apr.


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Identification of Relaxase-DNA Covalent Complexes and DNA Strand Transfer Reaction Products by Polyacrylamide Gel Electrophoresis.

Identification of Relaxase-DNA Covalent Complexes and DNA Strand Transfer Reaction Products by Polyacrylamide Gel Electrophoresis.

Lucas, M. and Moncalian, G.

Methods Mol Biol. 2075:145-156. doi: 10.1007/978-1-4939-9877-7

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Directed evolution of a bacterial WS/DGAT acyltransferase: improving tDGAT from Thermomonospora curvata.

Santín, O.; Galie, S. and Moncalián, G.

​Protein Eng Des Sel. 32:25-32. doi: 10.1093/protein/gzz011.

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fabH deletion increases DHA production in Escherichia coli expressing Pfa genes.

Giner-Robles, L.; Lázaro, B.; de la Cruz, F. and Moncalián, G.

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Loading of malonyl-CoA onto tandem acyl carrier protein domains of polyunsaturated fatty acid synthases.

Santín, O. and Moncalián, G.

J Biol Chem. 2018 Aug 10;293(32):12491-12501. doi: 10.1074/jbc.RA118.002443.

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Heterologous Expression of a Thermophilic Diacylglycerol Acyltransferase Triggers Triglyceride Accumulation in Escherichia coli.

Lázaro, B.; Villa, J.A.; Santín, O.; Cabezas, M.; Milagre, C.D.F.; de la Cruz, F. and Moncalián, G.

​Plos One. 12(4):e0176520. doi: 10.1371/journal.pone.0176520. 

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Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1.

Juárez A, Villa JA, Lanza VF, Lázaro B, de la Cruz F, Álvarez HM and Moncalián G.

Microbial Cell Factories 16,35. doi: 10.1186/s12934-017-0651-7.

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Relaxosomes and plasmid transfer in Gram-negative bacteria.

Zechner, E.L.; Moncalián, G. and de la Cruz, F.

Current Topics in Microbiology and Immunology. 413:93-113. doi: 10.1007/978-3-319-75241-9_4.

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Design of Novel Relaxase Substrates Based on Rolling Circle Replicases for Bioconjugation to DNA Nanostructures.

Sagredo S, de la Cruz F and Moncalián G.

PLoS One;11:e0152666. doi:0.1371/journal.pone.0152666.

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Orthogonal protein assembly on DNA nanostructures using relaxases.

Sagredo,S., Pirzer,T., Rafat, A.A., Goetzfried,M.A., Moncalian, G., Simmel, F.C. and de la Cruz, F.

Sagredo,S., Pirzer,T., Rafat, A.A., Goetzfried,M.A., Moncalian, G., Simmel, F.C. and de la Cruz, F. (2016b).  Orthogonal protein assembly on DNA nanostructures using relaxases. Angewandte Chemie Int. Ed. 55, 4348-52. doi: 10.1002/anie.201510313. IF 11.994. D1 (13/166)

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Comparative genomics of the conjugation region of F-like plasmids: five shades of F.

Fernandez Lopez R, De Toro Hernando M, Moncalian G, Garcillán-Barcia MP and de la Cruz F

Fernandez Lopez R, De Toro Hernando M, Moncalian G, Garcillán-Barcia MP and de la Cruz F (2016) Comparative genomics of the conjugation region of F-like plasmids: five shades of F. Front Mol Biosci. doi: 10.3389/fmolb.2016.00071

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Concerted action of NIC relaxase and auxiliary protein MobC in RA3 plasmid conjugation.

Godziszewska J, Moncalián G, Cabezas M, Bartosik AA, de la Cruz F, Jagura-Burdzy G. Mol Microbiol. 2016 Aug;101(3):439-56. doi: 10.1111/mmi.13401. Epub 2016 Jun 2.

[PubMed]

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Concerted action of NIC relaxase and auxiliary protein MobC in RA3 plasmid conjugation.

Godziszewska J, Moncalián G, Cabezas M, Bartosik AA, de la Cruz F and Jagura-Burdzy G.

Godziszewska J, Moncalián G, Cabezas M, Bartosik AA, de la Cruz F and Jagura-Burdzy G. (2016). Concerted action of NIC relaxase and auxiliary protein MobC in RA3 plasmid conjugation. Mol Microbiol. doi: 10.1111/mmi.13401.

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Effect of cerulenin on fatty acid composition and gene expression pattern of DHA-producing strain Colwellia psychrerythraea strain 34H.

Wan, X.; Peng, Y-F.; Zhou, X-R.; Gong, Y-M.; Huang, F-H. and Moncalian, G.

Wan, X.; Peng, Y-F.; Zhou, X-R.; Gong, Y-M.; Huang, F-H. and Moncalian, G. (2016). Effect of cerulenin on fatty acid composition and gene expression pattern of DHA-producing strain Colwellia psychrerythraea strain 34H. Microbial Cell Factories  15, 30. doi: 10.1186/s12934-016-0431-9.

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Physiological and genetic differences among Rhodococcus species for using glycerol as a source for growth and triacylglycerol production.

Herrero, M.O, Moncalian, G. and Alvarez, H.M.

Herrero, M.O, Moncalian, G. and Alvarez, H.M.(2016). Physiological and genetic differences among Rhodococcus species for using glycerol as a source for growth and triacylglycerol production.  Microbiology 162, 384-97. doi: 10.1099/mic.0.000232. 

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Transcription factor-based biosensors enlightened by the analyte.

Fernández-López R, Ruiz R, de la Cruz F, Moncalián G.

Fernández-López R, Ruiz R, de la Cruz F, Moncalián G. (2015) Transcription factor-based biosensors enlightened by the analyte. Front Microbiol. 6:648. doi: 10.3389/fmicb.2015.00648. 

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A high security double lock and key mechanism in HUH relaxases controls oriT-processing for plasmid conjugation.

Carballeira JD, González-Pérez B, Moncalián G, de la Cruz F. Nucleic Acids Res. 2014;42(16):10632-43. doi: 10.1093/nar/gku741.

[pubmed]

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Use of limited proteolysis and mutagenesis to identify folding domains and sequence motifs critical for wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase activity.

Villa JA, Cabezas M, de la Cruz F, Moncalián G. Appl Environ Microbiol. 2014 Feb;80(3):1132-41. doi: 10.1128/AEM.03433-13.

[pubmed]

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Use of limited proteolysis and mutagenesis to identify folding domains and sequence motifs critical for wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase activity.

Villa JA, Cabezas M, de la Cruz F, Moncalián G. Appl Environ Microbiol. 2014 Feb;80(3):1132-41. doi: 10.1128/AEM.03433-13.

[pubmed]

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Breaking and joining single-stranded DNA: the HUH endonuclease superfamily.

Chandler M, de la Cruz F, Dyda F, Hickman AB, Moncalian G, Ton-Hoang B. Nat Rev Microbiol. 2013 Aug;11(8):525-38. doi: 10.1038/nrmicro3067.

[pubmed]

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Catalytic domain of plasmid pAD1 relaxase TraX defines a group of relaxases related to restriction endonucleases.

Francia MV, Clewell DB, de la Cruz F, Moncalián G. Proc Natl Acad Sci U S A. 2013 Aug 13;110(33):13606-11. doi: 10.1073/pnas.1310037110. Epub 2013 Jul 31.

[pubmed]

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Interaction between relaxase MbeA and accessory protein MbeC of the conjugally mobilizable plasmid ColE1.

Varsaki A, Lamb HK, Eleftheriadou O, Vandera E, Thompson P, Moncalián G, de la Cruz F, Hawkins AR, Drainas C. FEBS Lett. 2012 Mar 23;586(6):675-9.

[pubmed]

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Relaxase DNA binding and cleavage are two distinguishable steps in conjugative DNA processing that involve different sequence elements of the nic site.

Lucas M, Gonzalez-Perez B, Cabezas M, Moncalián G, Rivas G, de la Cruz F. J Biol Chem. 2010 Mar 19; 285(12):8918-26. doi: 10.1074/jbc.M109.057539

[PubMed]

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Adrián Alonso Núñez

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