Inglés (Estados Unidos)

Gabriel Moncalián (IP)

IBBTEC. Albert Einstein, 22; 39011 Santander (Spain)
gabriel.moncalian@unican.es; maria.lucas@unican.es
(+34) 942 201934
Protein Design and Engineering
Principal Investigator
Structural Biology
Department of Microbiology & Genomics

Predoc (1994-2000): BSc in Chemical Sciences by the University of Valladolid. Doctoral thesis on the nucleoprotein complex that initiates bacterial conjugation supervised by Dr. Fernando de la Cruz in the Department of Molecular Biology of the University of Cantabria thanks to a MEC fellowship, with short term stays in the laboratories of Dr. Robert T. Sauer at MIT (Cambridge, MA) and Dr. Ellen Zechner at the University of Graz (Austria). Extraordinary doctorate award.

Postdoc 1 (2000-2003): Supervised by Dr. John A. Tainer at the Scripps Research Institute (La Jolla, CA) working on structural biology of DNA Repair.

Postdoc 2 (2003-2004): Supervised by Dr. Jerónimo Bravo at the CNIO (Madrid) working on structural biology of endocytosis.

Ramón y Cajal Contract (2005-2009): Reincorporation to the UC. Start of an independent project on the synthesis of triglycerides and polyunsaturated fatty acids.

Present: Associate Professor of Genetics at the University of Cantabria since 2009 and Principal Investigator of the Protein Design and Engineering Research Group at the Institute of Biomedicine and Biotechnology of Cantabria (IBBTEC) since 2014. In this last stage I have consolidated my research group. PI of different public national research projects and projects with national companies, highlighting the contract with Sniace to develop a zenith-E project for the improvement of bioethanol production. Regular user of the ALBA synchrotron. Coordinator of the master's and doctoral programs in Molecular Biology and Biomedicine and vice-dean of the Faculty of Medicine. I have published 40 articles in international journals (Nature, Cell, NSMB, PNAS, EMBOJ, Angewandte Chemie, etc), a patent for the production of triglycerides in bacteria and I have supervised eight doctoral theses.

Protein Design and Engineering

PIs: Gabriel Moncalián & María Lucas Gay

Research Lines

Structure, function and modification of polyunsaturated fatty acid synthases.
Optimization of the microbial production of added value fatty acids and triglycerides.
Optimization of proteins involved in bacterial conjugation for biotechnological applications.
Structure and function of protein complexes involved in intracellular vesicular traffic.
Molecular and structural basis of neurodegenerative diseases.
Structural basis of Host-Pathogen Interactions.

Services offered

Design and optimization of proteins with biotechnological, biomedical or industrial interest.
Structural analysis of proteins by X-ray crystallography and in silico molecular threading.

Funding

EUNICE - European University for Customised Education. 101004083-EUNICE-EAC-A02-2
REUNICE RESEARCH WITH AND FOR SOCIETY IN EUNICE. H2020-IBA-SfS-2-2020-101035813
Plataforma de cromatografía acoplada a espectrometría de masas (EQC2021-007113-P)
Microscopio electronico de transmisión de ultima generacion para el análisis estructural de macromoleculas biologicas (EQC2021-007003-P)
Modificación de megasintasas para la producción de precursores químicos sostenibles (TED2021-129278B-I00)
Megasintasas de acidos grasos poliinsaturados: estructura, optimizacion y aplicaciones (PID2021-122164NB-I00)
Mecanismo de acción del complejo Retriever (PID2021-122611NB-I00)

Thraustochytrid PUFA synthase ER domains form a stable heterodimer

Gabriel Moncalián (IP), Nahuel Lofeudo

Journal of Structural Biology: X 2026 Dec

Prokaryotic PfaB is a terminal acyltransferase that determines the final PUFA product

Lofeudo N, Martín A, Jácome M, Wan X, Lucas M, Moncalián G.

Protein Sci. 2026 Mar;35(3):e70497.

Selective cargo and membrane recognition by SNX17 regulates its interaction with Retriever

Martín-González A, Méndez-Guzmán I, Zabala-Zearreta M, Quintanilla A, García-López A, Martínez-Lombardía E, Albesa-Jové D, Acosta JC, Lucas M.

EMBO Rep 26, 470–493 (2025).

MLDSR, the transcriptional regulator of the major lipid droplets protein MLDS, is controlled by long-chain fatty acids and contributes to the lipid-accumulating phenotype in oleaginous Rhodococcus strains

Hernández MA, Ledesma AE, Moncalián G, Alvarez HM.

FEBS J. 2024 Apr;291(7):1457-1482.

TOR complex 1 negatively regulates NDR kinase Cbk1 to control cell separation in budding yeast

Foltman M, Mendez I, Bech-Serra JJ, de la Torre C, Brace JL, Weiss EL, Lucas M, Queralt E, Sanchez-Diaz A.

PLoS Biol. 2023 Aug 30;21(8):e3002263.

Insights into the Binding Mode of Lipid A to the Anti-lipopolysaccharide Factor ALFPm3 from Penaeus monodon: An In Silico Study through MD Simulations

González-Fernández C, Öhlknecht C, Diem M, Escalona Y, Bringas E, Moncalián G, Oostenbrink C, Ortiz I.

J Chem Inf Model. 2023 Apr 24;63(8):2495-2504.

Structural basis of direct and inverted DNA sequence repeat recognition by helix-turn-helix transcription factors

Fernandez-Lopez R, Ruiz R, Del Campo I, Gonzalez-Montes L, Boer DR, de la Cruz F, Moncalian G.

Nucleic Acids Res. 2022 Nov 11;50(20):11938-11947

Characterization of the DNA Binding Domain of StbA, A Key Protein of A New Type of DNA Segregation System

Quèbre V, Del Campo I, Cuevas A, Siguier P, Rech J, Le PTN, Ton-Hoang B, Cornet F, Bouet JY, Moncalian G, de la Cruz F, Guynet C.

J Mol Biol. 2022 Oct 15;434(19):167752.

Integrated strategy for the separation of endotoxins from biofluids. LPS capture on newly synthesized protein

Basauri A., Fallanza M., Giner-Robles L., Fernandez-Lopez R., Moncalián G., de la Cruz F., Ortiz I.

Separation and Purification Technology. Volume 255, 15 January 2021, 117689

Reprogramming microorganisms for the biosynthesis of astaxanthin via metabolic engineering

Wan, X.; Zhou, X-R.; Moncalian, G.; Su, L.; Chen, W-C.; Zhu, H-Z.; Chen, D.; Gong, Y-M.; Huang, F-H. and Deng, Q-C.

Prog Lipid Res. 2021 Jan;81:101083. doi: 10.1016/j.plipres.2020.101083. Epub 2020 Dec 26.

Structure and Mechanism of the Ketosynthase-Chain Length Factor Didomain from a Prototypical Polyunsaturated Fatty Acid Synthase

Santín, O., Yuet, K., Khosla, C. and Moncalián, G.

Biochemistry. 2020 Dec 22;59(50):4735-4743. doi: 10.1021/acs.biochem.0c00785. Epub 2020 Dec 7.

Structural insights into Pseudomonas aeruginosaType six secretion system exported effector 8.

González-Magaña A, Sainz-Polo MÁ, Pretre G, Çapuni R, Lucas M, Altuna J, Montánchez I, Fucini P, Albesa-Jové D.

J Struct Biol. 2020 Dec 1;212(3):107651. doi: 10.1016/j.jsb.2020.107651. Epub 2020 Oct 20.

CDC42EP5/BORG3 modulates SEPT9 to promote actomyosin function, migration, and invasion

Aaron J. Farrugia, Javier Rodríguez, Jose L. Orgaz, María Lucas, Victoria Sanz-Moreno, Fernando Calvo

J Cell Biol (2020) 219 (9): e201912159. https://doi.org/10.1083/jcb.201912159. Epub 2020 Aug 14

Biochemical interactions between LPS and LPS-binding molecules

Basauri A, González-Fernández C, Fallanza M, Bringas E, Fernandez-Lopez R, Giner L, Moncalián G, de la Cruz F, Ortiz I.

Crit Rev Biotechnol. 2020 May;40(3):292-305. doi: 10.1080/07388551.2019.1709797. Epub 2020 Jan 13.

ArdC, a ssDNA-binding protein with a metalloprotease domain, overpasses the recipient hsdRMS restriction system broadening conjugation host range

Lorena González-Montes, Irene Del Campo, M Pilar Garcillán-Barcia, Fernando de la Cruz, Gabriel Moncalián

PLoS Genet. 2020 Apr 29;16(4):e1008750. doi: 10.1371/journal.pgen.1008750. eCollection 2020 Apr.

Identification of Relaxase-DNA Covalent Complexes and DNA Strand Transfer Reaction Products by Polyacrylamide Gel Electrophoresis.

Lucas, M. and Moncalian, G.

Methods Mol Biol. 2075:145-156. doi: 10.1007/978-1-4939-9877-7

Regulation of Human Hsc70 ATPase and Chaperone Activities by Apg2: Role of the Acidic Subdomain

Cabrera Y, Dublang L, Fernández-Higuero JA, Albesa-Jové D, Lucas M, Viguera AR, Guerin ME, Vilar JMG, Muga A, Moro F.

J Mol Biol. 2019 Jan 18; 431(2):444-461. doi: 10.1016/j.jmb.2018.11.026.

Directed evolution of a bacterial WS/DGAT acyltransferase: improving tDGAT from Thermomonospora curvata.

Santín, O.; Galie, S. and Moncalián, G.

Protein Eng Des Sel. 32:25-32. doi: 10.1093/protein/gzz011.

fabH deletion increases DHA production in Escherichia coli expressing Pfa genes.

Giner-Robles, L.; Lázaro, B.; de la Cruz, F. and Moncalián, G.

Loading of malonyl-CoA onto tandem acyl carrier protein domains of polyunsaturated fatty acid synthases.

Santín, O. and Moncalián, G.

J Biol Chem. 2018 Aug 10;293(32):12491-12501. doi: 10.1074/jbc.RA118.002443.

RavN is a member of a previously unrecognized group of Legionella pneumophila E3 ubiquitin ligases.

Lin YH, Lucas M, Evans TR, Abascal-Palacios G, Doms AG, Beauchene NA, Rojas AL, Hierro A, Machner MP.

[PubMed]

Molecular mechanism for the subversion of the retromer coat by the Legionella effector RidL.

Romano-Moreno M, Rojas AL, Williamson CD, Gershlick DC, Lucas M, Isupov MN, Bonifacino JS, Machner MP, Hierro A.Proc Natl Acad Sci U S A. 2017 Dec 26;114(52):E11151-E11160. doi:10.1073/pnas.1715361115

PubMed

Heterologous Expression of a Thermophilic Diacylglycerol Acyltransferase Triggers Triglyceride Accumulation in Escherichia coli.

Lázaro, B.; Villa, J.A.; Santín, O.; Cabezas, M.; Milagre, C.D.F.; de la Cruz, F. and Moncalián, G.

Plos One. 12(4):e0176520. doi: 10.1371/journal.pone.0176520.

Endosomal Trafficking: Retromer and Retriever Are Relatives in Recycling.

Gershlick DC, Lucas M.

Curr Biol. 2017 Nov 20;27(22):R1233-R1236.

doi: 10.1016/j.cub.2017.10.004

Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1.

Juárez A, Villa JA, Lanza VF, Lázaro B, de la Cruz F, Álvarez HM and Moncalián G.

Microbial Cell Factories 16,35. doi: 10.1186/s12934-017-0651-7.

Relaxosomes and plasmid transfer in Gram-negative bacteria.

Zechner, E.L.; Moncalián, G. and de la Cruz, F.

Current Topics in Microbiology and Immunology. 413:93-113. doi: 10.1007/978-3-319-75241-9_4.

Retromer

Lucas M, Hierro A. Curr Biol. 2017 Jul 24; 27(14):687-689. doi: 10.1016/j.cub.2017.05.072

[PubMed]

Structural Mechanism for Cargo Recognition by the Retromer Complex.

Lucas M, Gershlick DC, Vidaurrazaga A, Rojas AL, Bonifacino JS, Hierro A. Cell. 2016 Dec 1;167(6):1623-1635. doi: 10.1016/j.cell.2016.10.056

[PubMed]

Design of Novel Relaxase Substrates Based on Rolling Circle Replicases for Bioconjugation to DNA Nanostructures.

Sagredo S, de la Cruz F and Moncalián G.

PLoS One;11:e0152666. doi:0.1371/journal.pone.0152666.

Orthogonal protein assembly on DNA nanostructures using relaxases.

Sagredo,S., Pirzer,T., Rafat, A.A., Goetzfried,M.A., Moncalian, G., Simmel, F.C. and de la Cruz, F.

Sagredo,S., Pirzer,T., Rafat, A.A., Goetzfried,M.A., Moncalian, G., Simmel, F.C. and de la Cruz, F. (2016b). Orthogonal protein assembly on DNA nanostructures using relaxases. Angewandte Chemie Int. Ed. 55, 4348-52. doi: 10.1002/anie.201510313. IF 11.994. D1 (13/166)

Comparative genomics of the conjugation region of F-like plasmids: five shades of F.

Fernandez Lopez R, De Toro Hernando M, Moncalian G, Garcillán-Barcia MP and de la Cruz F

Fernandez Lopez R, De Toro Hernando M, Moncalian G, Garcillán-Barcia MP and de la Cruz F (2016) Comparative genomics of the conjugation region of F-like plasmids: five shades of F. Front Mol Biosci. doi: 10.3389/fmolb.2016.00071

Concerted action of NIC relaxase and auxiliary protein MobC in RA3 plasmid conjugation.

Godziszewska J, Moncalián G, Cabezas M, Bartosik AA, de la Cruz F, Jagura-Burdzy G. Mol Microbiol. 2016 Aug;101(3):439-56. doi: 10.1111/mmi.13401. Epub 2016 Jun 2.

[PubMed]

Concerted action of NIC relaxase and auxiliary protein MobC in RA3 plasmid conjugation.

Godziszewska J, Moncalián G, Cabezas M, Bartosik AA, de la Cruz F and Jagura-Burdzy G.

Godziszewska J, Moncalián G, Cabezas M, Bartosik AA, de la Cruz F and Jagura-Burdzy G. (2016). Concerted action of NIC relaxase and auxiliary protein MobC in RA3 plasmid conjugation. Mol Microbiol. doi: 10.1111/mmi.13401.

Effect of cerulenin on fatty acid composition and gene expression pattern of DHA-producing strain Colwellia psychrerythraea strain 34H.

Wan, X.; Peng, Y-F.; Zhou, X-R.; Gong, Y-M.; Huang, F-H. and Moncalian, G.

Wan, X.; Peng, Y-F.; Zhou, X-R.; Gong, Y-M.; Huang, F-H. and Moncalian, G. (2016). Effect of cerulenin on fatty acid composition and gene expression pattern of DHA-producing strain Colwellia psychrerythraea strain 34H. Microbial Cell Factories 15, 30. doi: 10.1186/s12934-016-0431-9.

Physiological and genetic differences among Rhodococcus species for using glycerol as a source for growth and triacylglycerol production.

Herrero, M.O, Moncalian, G. and Alvarez, H.M.

Herrero, M.O, Moncalian, G. and Alvarez, H.M.(2016). Physiological and genetic differences among Rhodococcus species for using glycerol as a source for growth and triacylglycerol production. Microbiology 162, 384-97. doi: 10.1099/mic.0.000232.

Transcription factor-based biosensors enlightened by the analyte.

Fernández-López R, Ruiz R, de la Cruz F, Moncalián G.

Fernández-López R, Ruiz R, de la Cruz F, Moncalián G. (2015) Transcription factor-based biosensors enlightened by the analyte. Front Microbiol. 6:648. doi: 10.3389/fmicb.2015.00648.

Structural basis for the recruitment and activation of the Legionella phospholipase VipD by the host GTPase Rab5.

Lucas M, Gaspar AH, Pallara C, Rojas AL, Fernández-Recio J, Machner MP, Hierro A. Proc Natl Acad Sci U S A. 2014, Aug 26; 111(34):E3514-23. doi: 10.1073/pnas.1405391111

[PubMed]

A high security double lock and key mechanism in HUH relaxases controls oriT-processing for plasmid conjugation.

Carballeira JD, González-Pérez B, Moncalián G, de la Cruz F. Nucleic Acids Res. 2014;42(16):10632-43. doi: 10.1093/nar/gku741.

[pubmed]

Use of limited proteolysis and mutagenesis to identify folding domains and sequence motifs critical for wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase activity.

Villa JA, Cabezas M, de la Cruz F, Moncalián G. Appl Environ Microbiol. 2014 Feb;80(3):1132-41. doi: 10.1128/AEM.03433-13.

[pubmed]

Use of limited proteolysis and mutagenesis to identify folding domains and sequence motifs critical for wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase activity.

Villa JA, Cabezas M, de la Cruz F, Moncalián G. Appl Environ Microbiol. 2014 Feb;80(3):1132-41. doi: 10.1128/AEM.03433-13.

[pubmed]

Breaking and joining single-stranded DNA: the HUH endonuclease superfamily.

Chandler M, de la Cruz F, Dyda F, Hickman AB, Moncalian G, Ton-Hoang B. Nat Rev Microbiol. 2013 Aug;11(8):525-38. doi: 10.1038/nrmicro3067.

[pubmed]

Catalytic domain of plasmid pAD1 relaxase TraX defines a group of relaxases related to restriction endonucleases.

Francia MV, Clewell DB, de la Cruz F, Moncalián G. Proc Natl Acad Sci U S A. 2013 Aug 13;110(33):13606-11. doi: 10.1073/pnas.1310037110. Epub 2013 Jul 31.

[pubmed]

Interaction between relaxase MbeA and accessory protein MbeC of the conjugally mobilizable plasmid ColE1.

Varsaki A, Lamb HK, Eleftheriadou O, Vandera E, Thompson P, Moncalián G, de la Cruz F, Hawkins AR, Drainas C. FEBS Lett. 2012 Mar 23;586(6):675-9.

[pubmed]

The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repair.

Lammens K, Bemeleit DJ, Möckel C, Clausing E, Schele A, Hartung S, Schiller CB, Lucas M, Angermüller C, Söding J, Sträßer K, Hopfner KP. Cell. 2011 Apr1; 145(1):54-66. doi: 10.1016/j.cell.2011.02.038

[PubMed]

Purification, crystallization and preliminary crystallographic analysis of the CBS domain protein MJ1004 from Methanocaldococcus jannaschii.

Oyenarte I, Lucas M, Gomez-García I, Martínez-Cruz LA. Acta Crystallogr Sect F, 2011 Mar 1; 67(3):318-24. doi: 10.1107/S1744309110053479

[PubMed]

Relaxase DNA binding and cleavage are two distinguishable steps in conjugative DNA processing that involve different sequence elements of the nic site.

Lucas M, Gonzalez-Perez B, Cabezas M, Moncalián G, Rivas G, de la Cruz F. J Biol Chem. 2010 Mar 19; 285(12):8918-26. doi: 10.1074/jbc.M109.057539

[PubMed]

Binding of S-Methyl-5'-Thioadenosine and S-Adenosyl-l-Methionine to Protein MJ0100 Triggers an Open-to-Closed Conformational Change in Its CBS Motif Pair.

Lucas M, Encinar JA, Arribas EA, Oyenarte I, García IG, Kortazar D, Fernández JA, Mato JM, Martínez-Chantar ML, Martínez-Cruz LA. J Mol Biol. 2010 feb 26; 396(3):800-20. doi: 10.1016/j.jmb.2009.12.012

[PubMed]

The CBS domain protein MJ0729 of Methanocaldococcus jannaschii is a thermostable protein with a pH-dependent self-oligomerization.

Martínez-Cruz LA, Encinar JA, Kortazar D, Prieto J, Gómez J, Fernández-Millán P, Lucas M, Arribas EA, Fernández JA, Martínez-Chantar ML, Mato JM, Neira JL. Biochemistry. 2009 Mar 31; 48(12):2760-76. doi: 10.1021/bi801920r

[PubMed]

Purification, crystallization and preliminary X-ray diffraction analysis of the CBS-domain pair from the Methanococcus jannaschii protein MJ0100.

Lucas M, Kortazar D, Astigarraga E, Fernández JA, Mato JM, Martínez-Chantar ML, Martínez-Cruz LA. Acta Crystallogr Sect F. 2008 Oct 1;64(10):936-41. doi: 10.1107/S1744309108027930

[PubMed]

Crystallization and preliminary crystallographic analysis of merohedrally twinned crystals of MJ0729, a CBS-doman protein from Methanococcus jannaschii.

Fernández-Millán P, Kortazar D, Lucas M, Martínez-Chantar ML, Astigarraga-Arribas E, Fernández JA, Albert A, Mato JM, Martínez-Cruz LA.

Acta Crystallogr Sect F. 2008 Jul 1;64(7):605-9. [PubMed]

María Lucas Gay (IP)

Pedro Albendea Pérez

Pablo Duque Dorado

Nahuel Ismael Lofeudo Álvarez

Iván Méndez Guzmán

IBBTEC

Instituto de Biomedicina y Biotecnología de Cantabria.
PCTCAN - Cl. Albert Einstein, 22
39011 Santander
ibbtec@unican.es

Gabriel Moncalián (IP)

​​​​​​​​​​​​​Protein Design and Engineering

​​PIs: Gabriel Moncalián & María Lucas Gay

Research Lines

Services offered

Funding

Thraustochytrid PUFA synthase ER domains form a stable heterodimer

Prokaryotic PfaB is a terminal acyltransferase that determines the final PUFA product

Selective cargo and membrane recognition by SNX17 regulates its interaction with Retriever

MLDSR, the transcriptional regulator of the major lipid droplets protein MLDS, is controlled by long-chain fatty acids and contributes to the lipid-accumulating phenotype in oleaginous Rhodococcus strains

TOR complex 1 negatively regulates NDR kinase Cbk1 to control cell separation in budding yeast

Insights into the Binding Mode of Lipid A to the Anti-lipopolysaccharide Factor ALFPm3 from Penaeus monodon: An In Silico Study through MD Simulations

Structural basis of direct and inverted DNA sequence repeat recognition by helix-turn-helix transcription factors

Characterization of the DNA Binding Domain of StbA, A Key Protein of A New Type of DNA Segregation System

Integrated strategy for the separation of endotoxins from biofluids. LPS capture on newly synthesized protein

Reprogramming microorganisms for the biosynthesis of astaxanthin via metabolic engineering

Structure and Mechanism of the Ketosynthase-Chain Length Factor Didomain from a Prototypical Polyunsaturated Fatty Acid Synthase

Structural insights into Pseudomonas aeruginosaType six secretion system exported effector 8.

CDC42EP5/BORG3 modulates SEPT9 to promote actomyosin function, migration, and invasion

Biochemical interactions between LPS and LPS-binding molecules

ArdC, a ssDNA-binding protein with a metalloprotease domain, overpasses the recipient hsdRMS restriction system broadening conjugation host range

Identification of Relaxase-DNA Covalent Complexes and DNA Strand Transfer Reaction Products by Polyacrylamide Gel Electrophoresis.

Regulation of Human Hsc70 ATPase and Chaperone Activities by Apg2: Role of the Acidic Subdomain

Directed evolution of a bacterial WS/DGAT acyltransferase: improving tDGAT from Thermomonospora curvata.

fabH deletion increases DHA production in Escherichia coli expressing Pfa genes.

Loading of malonyl-CoA onto tandem acyl carrier protein domains of polyunsaturated fatty acid synthases.

RavN is a member of a previously unrecognized group of Legionella pneumophila E3 ubiquitin ligases.

Molecular mechanism for the subversion of the retromer coat by the Legionella effector RidL.

Heterologous Expression of a Thermophilic Diacylglycerol Acyltransferase Triggers Triglyceride Accumulation in Escherichia coli.

Endosomal Trafficking: Retromer and Retriever Are Relatives in Recycling.

Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1.

Relaxosomes and plasmid transfer in Gram-negative bacteria.

Retromer

Structural Mechanism for Cargo Recognition by the Retromer Complex.

Design of Novel Relaxase Substrates Based on Rolling Circle Replicases for Bioconjugation to DNA Nanostructures.

Orthogonal protein assembly on DNA nanostructures using relaxases.

Comparative genomics of the conjugation region of F-like plasmids: five shades of F.

Concerted action of NIC relaxase and auxiliary protein MobC in RA3 plasmid conjugation.

Concerted action of NIC relaxase and auxiliary protein MobC in RA3 plasmid conjugation.

Effect of cerulenin on fatty acid composition and gene expression pattern of DHA-producing strain Colwellia psychrerythraea strain 34H.

Physiological and genetic differences among Rhodococcus species for using glycerol as a source for growth and triacylglycerol production.

Transcription factor-based biosensors enlightened by the analyte.

Structural basis for the recruitment and activation of the Legionella phospholipase VipD by the host GTPase Rab5.

A high security double lock and key mechanism in HUH relaxases controls oriT-processing for plasmid conjugation.

Use of limited proteolysis and mutagenesis to identify folding domains and sequence motifs critical for wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase activity.

Use of limited proteolysis and mutagenesis to identify folding domains and sequence motifs critical for wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase activity.

Breaking and joining single-stranded DNA: the HUH endonuclease superfamily.

Catalytic domain of plasmid pAD1 relaxase TraX defines a group of relaxases related to restriction endonucleases.

Interaction between relaxase MbeA and accessory protein MbeC of the conjugally mobilizable plasmid ColE1.

The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repair.

Purification, crystallization and preliminary crystallographic analysis of the CBS domain protein MJ1004 from Methanocaldococcus jannaschii.

Relaxase DNA binding and cleavage are two distinguishable steps in conjugative DNA processing that involve different sequence elements of the nic site.

Binding of S-Methyl-5'-Thioadenosine and S-Adenosyl-l-Methionine to Protein MJ0100 Triggers an Open-to-Closed Conformational Change in Its CBS Motif Pair.

The CBS domain protein MJ0729 of Methanocaldococcus jannaschii is a thermostable protein with a pH-dependent self-oligomerization.

Purification, crystallization and preliminary X-ray diffraction analysis of the CBS-domain pair from the Methanococcus jannaschii protein MJ0100.

Crystallization and preliminary crystallographic analysis of merohedrally twinned crystals of MJ0729, a CBS-doman protein from Methanococcus jannaschii.

María Lucas Gay (IP)

Pedro Albendea Pérez

Pablo Duque Dorado

Nahuel Ismael Lofeudo Álvarez

Iván Méndez Guzmán

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IBBTEC

Protein Design and Engineering

PIs: Gabriel Moncalián & María Lucas Gay