Iñaki Arechaga Iturregui (IP)

  • IBBTEC. Albert Einstein 22, PCTCAN, 39011 Santander
  • ignacio.arechaga@unican.es
  • 942 202033
  • Molecular Motors in Nanobiotechnology
  • Principal Investigator
  • Structural Biology
  • Department of Microbiology & Genomics

Ignacio se licenció en Ciencias Biológicas por la Universidad del País Vasco (UPV-EHU) (1990). Desarrolló su tesis doctoral en Biología Molecular en el Centro de Investigaciones Biológicas (CIB-CSIC), consiguiendo el Premio Extraordinario de Tesis Doctoral en la Universidad Autónoma de Madrid. Durante 10 años (1994-2004), realizó diversas estancias postdoctorales (MRC-Laboratory of Molecular Biology,  Cambridge, UK (1994-1999); EMBO, Long Term Fellowship (1994-1996), HFSP (1996-1999); MRC-Dunn Human Nutrition Centre, Cambridge, UK (1999-2003); Centro Nacional de Biotecnología, CNB-CSIC (2003-2004), después de las cuales consiguió una plaza como Investigador Ramón y Cajal (2004-2008), para posteriormente incorporarse a la Universidad de Cantabria, primero como Profesor Contratado Doctor (2008-2010), y actualmente como Profesor Titular de Universidad (2010--).

​Molecular Motors in Nanobiotechnology


Research interests

  • Structural and biochemical approach into the molecular mechanism of RecA/AAA+ motors (ATPases Associated to a large variety of cellular Activities (i.e. DNA unwinding, protein folding, DNA transport…) 
  • Inhibitors of bacterial conjugation to prevent the dissemination of antibiotic resistance genes

  • Protein nanopores for DNA sequencing and susbstrate secretion
  • Electron microscopy of large membrane protein complexes 

Funding

  • Título del proyecto: “Aplicaciones biomédicas y biotecnológicas de motores moleculares implicados en la transferencia de ADN y proteínas a través de membranas biológicas”
    Entidad financiadora:   Ministerio de Economía, Industria y Competitividad
    Referencia: BFU2016-78521-R
    Duración:desde 30/12/2016 hasta 29/12/2019
    Investigadores responsables: Dr. Elena Cabezón e Ignacio Arechaga

  • Título del proyecto: "Aplicación de un motor que transloca ssDNA en plataformas de secuenciación de tercera generación"
    Entidad financiadora: Ministerio de Economía y Competitividad
    Referencia: BFU2014-61823-EXP
    Duración: desde 01/09/2015 hasta 31/08/2017
    Investigador responsable: Dr. Elena Cabezón

  • Título del proyecto: "Morfogénesis de membranas bacterianas y mitocondriales: en busca del eslabón perdido"

    Entidad financiadora: Ministerio de Economía y Competitividad 

    Referencia : BFU2013_49486-EXP

    Duración: 2014-2016

    Investigador responsable: Dr. Ignacio Arechaga

  • Título del proyecto: "Mecanismos moleculares y evolutivos en motores moleculares que translocan ADN y proteínas"

    Entidad financiadora: Ministerio de Economía y Competitividad

    Referencia: BFU2011-22874

    Duración:desde 01/01/2012 hasta 31/12/2015 

    Investigador responsable: Dr. Elena Cabezón

Primary architecture and energy requirements of Type III and Type IV secretion systems

Primary architecture and energy requirements of Type III and Type IV secretion systems

Cabezón E, Valenzuela-Gómez F, Arechaga I.

​​Front Cell Infect Microbiol. 2023 Nov 27;13:1255852.​

View more

Nanopore sensing reveals a preferential pathway for the co-translocational unfolding of a conjugative relaxase-DNA complex

Nanopore sensing reveals a preferential pathway for the co-translocational unfolding of a conjugative relaxase-DNA complex

Valenzuela-Gómez F, Arechaga I, Cabezón E.

Nucleic Acids Res. 2023 Jun 2:gkad492. Online ahead of print.​​

View more

Monitoring Bacterial Conjugation by Optical Microscopy

Monitoring Bacterial Conjugation by Optical Microscopy

Carranza G, Menguiano T, Valenzuela-Gómez F, García-Cazorla Y, Cabezón E, Arechaga I.

Front Microbiol. 2021 Oct 4;12:750200.

View more

Drug Weaponry to Fight Against SARS-CoV-2

Drug Weaponry to Fight Against SARS-CoV-2

Cabezón E, Arechaga I.

Front Mol Biosci. 2020 Aug 25;7:204.

View more

Myc stimulates cell cycle progression through the activation of Cdk1 and phosphorylation of p27

Myc stimulates cell cycle progression through the activation of Cdk1 and phosphorylation of p27

García-Gutiérrez L, Bretones G, Molina E, Arechaga I, Symonds C, Acosta JC, Blanco R, Fernández A, Alonso L, Sicinski P, Barbacid M, Santamaría D, León J.

​Sci Rep. 2019 Dec 10;9(1):18693. doi: 10.1038/s41598-019-54917-1.

View more

Spectrophotometric Assays to Quantify the Activity of T4SS ATPases

Spectrophotometric Assays to Quantify the Activity of T4SS ATPases

Cabezón E, Arechaga I.

​In: de la Cruz, F. (eds) Horizontal Gene Transfer. Methods in Molecular Biology, vol 2075. Humana, New York, NY., 2019

View more

The FtsK-like motor TraB is a DNA-dependent ATPase that forms higher-order assemblies

The FtsK-like motor TraB is a DNA-dependent ATPase that forms higher-order assemblies

Amado E, Muth G, Arechaga I, Cabezón E.

​J Biol Chem. 2019 Mar 29;294(13):5050-5059.

View more

Conjugation inhibitors compete with palmitic acid for binding to the conjugative traffic ATPase TrwD, providing a mechanism to inhibit bacterial conjugation

Conjugation inhibitors compete with palmitic acid for binding to the conjugative traffic ATPase TrwD, providing a mechanism to inhibit bacterial conjugation

García-Cazorla Y, Getino M, Sanabria-Ríos DJ, Carballeira NM, de la Cruz F, Arechaga I, Cabezón E

​J Biol Chem. 2018 Oct 26;293(43):16923-16930. 

View more

Conjugation Inhibitors and Their Potential Use to Prevent Dissemination of Antibiotic Resistance Genes in Bacteria

Conjugation Inhibitors and Their Potential Use to Prevent Dissemination of Antibiotic Resistance Genes in Bacteria

Cabezón E, de la Cruz F, Arechaga I.

​Front Microbiol. 2017 Nov 30;8:2329. doi: 10.3389/fmicb.2017.02329. eCollection 2017.

View more

Type IV traffic ATPase TrwD as molecular target to inhibit bacterial conjugation.

Ripoll-Rozada J, García-Cazorla Y, Getino M, Machón C, Sanabria-Ríos D, de la Cruz F, Cabezón E, Arechaga I. Mol Microbiol. 2016 Jun;100(5):912-21. doi: 10.1111/mmi.13359.

[pubmed]

View more

Type IV traffic ATPase TrwD as molecular target to inhibit bacterial conjugation.

Ripoll-Rozada J, García-Cazorla Y, Getino M, Machón C, Sanabria-Ríos D, de la Cruz F, Cabezón E, Arechaga I. Mol Microbiol. 2016 Jun;100(5):912-21. doi: 10.1111/mmi.13359. Epub 2016 Mar 22.

[PubMed]

View more

Towards an integrated model of bacterial conjugation.

Cabezón E, Ripoll-Rozada J, Peña A, de la Cruz F, Arechaga I. FEMS Microbiol Rev. Vol.39:81-95. doi: 10.1111/1574-6976.12085.

 [pubmed]

View more

Functional interactions of VirB11 traffic ATPases with VirB4 and VirD4 molecular motors in type IV secretion systems.

Ripoll-Rozada J, Zunzunegui S, de la Cruz F, Arechaga I, Cabezón E. J Bacteriol. 2013 Sep;195(18):4195-201. doi: 10.1128/JB.00437-13.

[pubmed]

View more

Molecular motors in bacterial secretion.

Peña A, Arechaga I. J Mol Microbiol Biotechnol. 2013;23(4-5):357-69. doi: 10.1159/000351360.

[pubmed]

View more

Structural independence of conjugative coupling protein TrwB from its Type IV secretion machinery

Larrea D, de Paz HD, Arechaga I, de la Cruz F, Llosa M. Plasmid. 2013 Jul;70(1):146-53. doi: 10.1016/j.plasmid.2013.03.006.

[pubmed]

View more

Membrane invaginations in bacteria and mitochondria: common features and evolutionary scenarios.

Arechaga I. J Mol Microbiol Biotechnol. 2013;23(1-2):13-23. doi: 10.1074/jbc.M112.413849.

 [pubmed]

View more

The hexameric structure of a conjugative VirB4 protein ATPase provides new insights for a functional and phylogenetic relationship with DNA translocases.

Peña A, Martín-Benito J, Valpuesta JM, Carrascosa JL, de la Cruz F, Cabezón E*, Arechaga I*. J. Biol. Chem. 2012, 287(47), 39925-32. doi: 10.1074/jbc.M112.413849.

[pubmed]

View more

Regulation of the type IV secretion ATPase TrwD by magnesium: implications for the catalytic mechanism of the secretion ATPase superfamily.

Ripoll-Rozada J, Peña A, Rivas S, Moro F, de la Cruz F, Cabezón E*,Arechaga I* J. Biol. Chem. 2012, 287(21), 17408-14. DOI:10.1074/jbc.M112.357905.

[pubmed]

View more

Membrane-associated nanomotors form macromolecular transport.

Cabezón E*, Lanza VF, Arechaga I* Curr. Opin. Biotechnol. 2012, 23(4), 537-44. DOI:10.1016/j.copbio.2011.11.031.

​[pubmed]

View more

Increased sensitivity of antigen-experienced T cells through the enrichment of oligomeric T cell receptor complexes Immunity.

Kumar R, Ferez M, Swamy M, Arechaga I, Rejas MT, Valpuesta JM, Schamel WW, Alarcón B, van Santen HM Immunity 2011, 35(3), 375-87. DOI:10.1016/j.immuni.2011.08.010.

​[pubmed]

View more

Autoinhibitory regulation of TrwK, an essential VirB4 ATPase in type IV secretion systems.

Peña A, Ripoll-Rozada J, Zunzunegui S, Cabezón E, de la Cruz F, Arechaga I* J. Biol. Chem. 2011, 286(19), 17376-82. DOI:10.1074/jbc.M110.208942.

​[pubmed]

View more

Detection and functional characterization of a 215 amino acid N-terminal extension in the Xanthomonas type III effector XopD.

Canone J, Marino D, Noel LD, Arechaga I, Pichereaux C, Rossignol M, Roby D, Rivas S* PLOS One 2010, 22(5), e15773. DOI:10.1371/journal.pone.0015773.

​[pubmed]

View more

Structural characterization of the TCR complex by electron microscopy.

Arechaga I, Swamy M, Abia D, Schamel WW, Alarcón B, Valpuesta JM* Int. Immunol. 2010, 22(11), 897-903. DOI:10.1093/intimm/dxq443.

​[pubmed]

View more

Electron microscopy analysis of mammalian phosphofructokinase reveals an unusual 3-dimensional structure with significant implications for enzyme function.

Arechaga I, Martínez-Costa OH, Ferreras C, Carrascosa JL, Aragón JJ* FASEB J. 2010, 24(12), 4960-8. DOI:10.0024-4960.

​[pubmed]

View more

Elena Cabezón Navarro (IP)

View more

Tamara Menguiano Vázquez

View more