Matxalen Llosa Blas (IP)

  • IBBTEC. Albert Einstein 22, PCTCAN, 39011 Santande​r
  • matxalen.llosa@unican.es
  • 942 201957
  • Bacterial Type IV Secretion Systems
  • Principal Investigator
  • Microbiology & Genomics
  • Department of Microbiology & Genomics

Matxalen Llosa studied Biology at the University of Sevilla and got her PhD in Biological Sciences at the University of Cantabria, Spain. After postdoctoral research training periods at Brown University (USA) and the University of California at Berkeley (USA), she returned to the University of Cantabria as Associate Professor in 1998. Since 2003, she leads a research team focused on the biology of Type IV Secretion Systems, which was selected with 10 other teams to launch the new Cantabrian Institute for Biomedicine and Biotechnology (IBBTEC) in 2007. She is currently a Full Professor in Genetics at the University of Cantabria. She recently spent a sabbatical year in Yale University. Her research team aims to decipher the molecular mechanism of substrate recruitment and DNA transfer by Type IV Secretion Systems, and to develop biotechnological tools based on these systems to transfer proteins and DNA into different cellular types, including specific human cells in vivo.

​Biology of Bacterial Type IV Secretion Systems

Host group: Dra. Ana Villar


Research lines 

    •  Use of bacterial secretion systems for the genomic modification of human cells
    •  Site-specific recombination and integration catalyzed by conjugative relaxases 
    •  Substrate Recruitment by Type IV Secretion systems 

Funding:

    • MINECO BIO2017-87190-R, “Secreción de ADN de bacterias a células de mamífero. Análisis de su papel en virulencia y su potencial aplicación terapéutica”. IP: Matxalen Llosa
    • AECC IDEAS211LLOS, "Papel de la secreción de ADN bacteriano en el desarrollo tumoral". IP: Matxalen Llosa
    • SODERCAN/FEDER JU06. “Reclutamiento de Proteínas y ADN por sistemas de secreción Tipo IV A y B”. IP: Matxalen Llosa

Lab Retreat 2019

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Lab Retreat 2018

 







Conjugative DNA transfer from E. coli to transformation-resistant lactobacilli

Samperio S, Guzmán-Herrador DL, May-Cuz R, Martín MC, Álvarez MA, Llosa M.

​Frontiers in Microbiology, feb 2021, in press. doi: 10.3389/fmicb.2021.606629

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Editorial

Editorial

Susana Brom, Elisabeth Grohman, Matxalen Llosa

Plasmid. 2021 Jan;113:102526. doi: 10.1016/j.plasmid.2020.102526.


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Bacterial injection machines: Evolutionary diverse but functionally convergent.

Bacterial injection machines: Evolutionary diverse but functionally convergent.

Bleves S, Galán JE, Llosa M.

Cell Microbiol. 2020 May;22(5):e13157. doi: 10.1111/cmi.13157. 

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The secret life of conjugative relaxases

The secret life of conjugative relaxases

Dolores L. Guzmán-Herrador and Matxalen Llosa

Plasmid. 2019 Jul;104:102415. doi: 10.1016/j.plasmid.2019.102415.

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Exchange of functional domains between a bacterial conjugative relaxase and the integrase of the human adeno-associated virus

Leticia Agundez, Francisco Zarate-Perez, Anita F. Meier, Martino Bardelli, Matxalen Llosa, Carlos R. Escalante, R. Michael Linden, Els Henckaerts

​PLoS ONE 13 (7): e0200841. https://doi.org/10.1371/journal.pone.0200841

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DNA Delivery and Genomic Integration into Mammalian Target Cells through Type IV A and B Secretion Systems of Human Pathogens

Dolores L. Guzmán-Herrador, Samuel Steiner, Anabel Alperi, Coral González-Prieto†, Craig R. Roy and Matxalen Llosa*

​Front. Microbiol. 2017, 8:1503.doi: 10.3389/fmicb.2017.01503

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Substrate translocation involves specific lysine residues of the central channel of the conjugative coupling protein TrwB.

Larrea D, de Paz HD, Matilla I, Guzmán-Herrador DL, Lasso G, de la Cruz F, Cabezón E, Llosa M. ol Genet Genomics. 2017 Jun 8. doi: 10.1007/s00438-017-1331-3. (Epub ahead of print)

[pubmed]

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The Conjugative Relaxase TrwC Promotes Integration of Foreign DNA in the Human Genome.

González-Prieto C, Gabriel R, Dehio C, Schmidt M, Llosa M. Appl Environ Microbiol, 83:e00207-17. doi: 10.1128/AEM.00207-17. [Epub ahead of print]

[pubmed]

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A Functional oriT in the Ptw Plasmid of Burkholderia cenocepacia Can Be Recognized by the R388 Relaxase TrwC.

Fernández-González E, Bakioui S, Gomes MC, O'Callaghan D, Vergunst AC, Sangari FJ, Llosa M. Front Mol Biosci. 2016 May 3;3:16. doi: 10.3389/fmolb.2016.00016. eCollection 2016.

[pubmed]

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Chloramphenicol Selection of IS10 Transposition in the cat Promoter Region of Widely Used Cloning Vectors.

González-Prieto C, Agúndez L, Llosa M. PLoS One. 2015 Sep 16;10(9):e0138615. doi: 10.1371/journal.pone.0138615. eCollection 2015.

[pubmed]

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A translocation motif in relaxase TrwC specifically affects recruitment by its conjugative type IV secretion system.

Alperi A, Larrea D, Fernández-González E, Dehio C, Zechner EL, Llosa M. J Bacteriol. 2013 Nov;195(22):4999-5006. doi: 10.1128/JB.00367-13. Epub 2013 Aug 30.

[pubmed]

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Structural independence of conjugative coupling protein TrwB from its Type IV secretion machinery

Larrea D, de Paz HD, Arechaga I, de la Cruz F, Llosa M. Plasmid. 2013 Jul;70(1):146-53. doi: 10.1016/j.plasmid.2013.03.006.

[pubmed]

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HUH site-specific recombinases for targeted modification of the human genome.

González-Prieto C, Agúndez L, Linden RM, Llosa M. Trends Biotechnol. 2013 May;31(5):305-12. doi: 10.1016/j.tibtech.2013.02.002. Epub 2013 Mar 30. Review.

 [pubmed]

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New perspectives into bacterial DNA transfer to human cells.

Llosa M, Schröder G, Dehio C. Trends Microbiol. 2012 Aug;20(8):355-9. doi: 10.1016/j.tim.2012.05.008. Epub 2012 Jun 29. Review.

[pubmed]

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Site-specific integration of foreign DNA into minimal bacterial and human target sequences mediated by a conjugative relaxase.

Agúndez L, González-Prieto C, Machón C, Llosa M. PLoS One. 2012;7(1):e31047. doi: 10.1371/journal.pone.0031047. Epub 2012 Jan 23.

[pubmed]

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Transfer of R388 derivatives by a pathogenesis-associated type IV secretion system into both bacteria and human cells

Fernández-González E, de Paz HD, Alperi A, Agúndez L, Faustmann M, Sangari FJ, Dehio C, Llosa M. J Bacteriol 193 (22), 6257–6265

​[pubmed]


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Dolores Lucía Guzmán Herrador

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Ana Palanca Cuñado

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