Raúl Fernández López (IP)

  • raul.fernandez@unican.es
  • 942 206 799 ext. 25932
  • Intergenomics
  • Principal Investigator
  • Microbiology & Genomics
  • Department of Microbiology & Genomics

​​​Intergenomics


Research lines

– Evolution and Ecology:

         • Classification of conjugative and mobilizable plasmids based on their relaxase sequences.

         • Conjugative systems of Cyanobacteria and Actinomycetes.  

– Molecular Engines: Protein and DNA transporters across bacterial membranes

– Protein Engineering: Conjugative relaxases as a target for the inhibition of the dissemination of antibiotic resistance.

– Systems Biology


Funding


- Nuevas estrategias para el control de infecciones nosocomiales. INNOECO RTC-2015-3184-1. 

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​ ​“Promover el desarrollo tecnológico, la innovación y una investigación de calidad”


- Plásmidos ortogonales para Biología Sintética y de Sistemas. Ministerio de Educación (BFU2011-26608).

Bacterial Computing with Engineered Populations (BACTOCOM). European Union 7th Framework. Programme, Area Information and Communication Technologies (FP7-ICT-2009-4; Proyecto nº 248919).

- EVOTAR: Evolution and transfer of antibiotic resistance (Project no. 282004). European Union FP7-HEALTH 2011.2.3.1-2.

- Diversidad y distribución de plásmidos de bacterias ácido-lácticas. Acción Integrada Hispano-Serbia. PRI-AIBSE-2011-1186. IP: Fernando de la Cruz

- INGENIERÍA GENÉTICA DE ESCHERICHIA COLI PARA MEJORAR LA PRODUCCIÓN DE LÍPIDOS RICOS EN ÁCIDOS GRASOS OMEGA-3 (TF16-XX-003), cofinanciado por SODERCAN S.A. y el Programa Operativo FEDER



Structural basis of direct and inverted DNA sequence repeat recognition by helix-turn-helix transcription factors

Structural basis of direct and inverted DNA sequence repeat recognition by helix-turn-helix transcription factors

Fernandez-Lopez R, Ruiz R, Del Campo I, Gonzalez-Montes L, Boer DR, de la Cruz F, Moncalian G.

​Nucleic Acids Res. 2022 Nov 11;50(20):11938-11947

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Isolation and phenotypic and genomic characterization of Tetragenococcus spp. from two Spanish traditional blue-veined cheeses made of raw milk

Isolation and phenotypic and genomic characterization of Tetragenococcus spp. from two Spanish traditional blue-veined cheeses made of raw milk

Rodríguez J, González-Guerra A, Vázquez L, Fernández-López R, Flórez AB, de la Cruz F, Mayo B.

​Int J Food Microbiol. 2022 Apr 8;371:109670.

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Conjugation Inhibitors Effectively Prevent Plasmid Transmission in Natural Environments

Palencia-Gándara C, Getino M, Moyano G, Redondo S, Fernández-López R, González-Zorn B, de la Cruz F.

mBio. 2021 Aug 31;12(4):e0127721.

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Integrated strategy for the separation of endotoxins from biofluids. LPS capture on newly synthesized protein

Integrated strategy for the separation of endotoxins from biofluids. LPS capture on newly synthesized protein

Basauri A., Fallanza M., Giner-Robles L., Fernandez-Lopez R., Moncalián G., de la Cruz F., Ortiz I.

Separation and Purification Technology. Volume 255, 15 January 2021, 117689



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Pathways for horizontal gene transfer in bacteria revealed by a global map of their plasmids

Santiago Redondo-Salvo, Raúl Fernández-López, Raúl Ruiz, Luis Vielva, María de Toro, Eduardo P C Rocha, M Pilar Garcillán-Barcia, Fernando de la Cruz

​Nat Commun. 2020 Jul 17;11(1):3602. doi: 10.1038/s41467-020-17278-2.


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Biochemical interactions between LPS and LPS-binding molecules

Basauri A, González-Fernández C, Fallanza M, Bringas E, Fernandez-Lopez R, Giner L, Moncalián G, de la Cruz F, Ortiz I.

​Crit Rev Biotechnol. 2020 May;40(3):292-305. doi: 10.1080/07388551.2019.1709797. Epub 2020 Jan 13.

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Comparative genomics of the conjugation region of F-like plasmids: five shades of F.

Fernandez Lopez R, De Toro Hernando M, Moncalian G, Garcillán-Barcia MP and de la Cruz F

Fernandez Lopez R, De Toro Hernando M, Moncalian G, Garcillán-Barcia MP and de la Cruz F (2016) Comparative genomics of the conjugation region of F-like plasmids: five shades of F. Front Mol Biosci. doi: 10.3389/fmolb.2016.00071

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Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells.

Okumus B, Landgraf D, Lai GC, Bakhsi S, Arias-Castro JC, Yildiz S, Huh D, Fernandez-Lopez R, Peterson CN, Toprak E, El Karoui M, Paulsson J. Nat Commun. 2016 May 18;7:11641. doi: 10.1038/ncomms11641.

[PubMed]

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Transcription factor-based biosensors enlightened by the analyte.

Fernández-López R, Ruiz R, de la Cruz F, Moncalián G.

Fernández-López R, Ruiz R, de la Cruz F, Moncalián G. (2015) Transcription factor-based biosensors enlightened by the analyte. Front Microbiol. 6:648. doi: 10.3389/fmicb.2015.00648. 

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Rebooting the genome: The role of negative feedback in horizontal gene transfer.

Fernandez-Lopez R, de la Cruz F. Mob Genet Elements. 2015 Feb 23;4(6):1-6. eCollection 2014 Nov-Dec.

[PubMed]

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Negative feedback and transcriptional overshooting in a regulatory network for horizontal gene transfer.

Fernandez-Lopez R, Del Campo I, Revilla C, Cuevas A, de la Cruz F. PLoS Genet. 2014 Feb 27;10(2):e1004171. doi: 10.1371/journal.pgen.1004171.

[pubmed]

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Miguel Baez Martín

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Marina Domínguez Quintero

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Sheila González Gutiérrez

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Raquel Gutiérrez Lanza

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Alfonso Mendaña Gomez

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