| Distinct serum proteome profiles associated with collagen-induced arthritis, and Complete Freund’s Adjuvant-induced inflammation in CD38-/- mice: the discriminative power of protein species or proteoforms. | | Distinct serum proteome profiles associated with collagen-induced arthritis, and Complete Freund’s Adjuvant-induced inflammation in CD38-/- mice: the discriminative power of protein species or proteoforms. | A. Rosal-Vela, S. García-Rodríguez, J. Postigo, M. Iglesias, J. Merino, R. Merino, M. Zubiaur and J. Sancho. | 2015-09-30T22:00:00Z | <p><strong class="ms-rteFontSize-2" style="text-align:justify;">Abstract:</strong></p><p style="text-align:justify;"><span class="ms-rteFontSize-2">Collagen-type-II-induced arthritis (CIA) is an autoimmune disease, which involves a complex host systemic response including inflammatory and autoimmune reactions. CIA is milder in </span><em class="ms-rteFontSize-2">CD38</em><sup class="ms-rteFontSize-2">-</sup><sup class="ms-rteFontSize-2"><em>/</em></sup><sup class="ms-rteFontSize-2">-</sup><span class="ms-rteFontSize-2">than in wild-type (WT) mice. ProteoMiner-equalized serum samples were subjected to 2D-DiGE and MS-MALDI-TOF/TOF analyses to identify proteins that changed in their relative abundances in </span><em class="ms-rteFontSize-2">CD38</em><sup class="ms-rteFontSize-2">-</sup><sup class="ms-rteFontSize-2"><em>/</em></sup><sup class="ms-rteFontSize-2">-</sup><span class="ms-rteFontSize-2">versus WT mice either with arthritis (CIA+), with no arthritis (CIA-), or with inflammation (complete Freund's adjuvant (CFA)-treated mice). Multivariate analyses revealed that a multiprotein signature (</span><em class="ms-rteFontSize-2">n</em><em class="ms-rteFontSize-2"> </em><span class="ms-rteFontSize-2">= 28) was able to discriminate CIA+ from CIA- mice, and WT from </span><em class="ms-rteFontSize-2">CD38</em><sup class="ms-rteFontSize-2">-</sup><sup class="ms-rteFontSize-2"><em>/</em></sup><sup class="ms-rteFontSize-2">-</sup><span class="ms-rteFontSize-2">mice within each condition. Likewise, a distinct multiprotein signature (</span><em class="ms-rteFontSize-2">n</em><em class="ms-rteFontSize-2"> </em><span class="ms-rteFontSize-2">= 16) was identified which differentiated CIA+ </span><em class="ms-rteFontSize-2">CD38</em><span class="ms-rteFontSize-2">−</span><em class="ms-rteFontSize-2">/</em><span class="ms-rteFontSize-2">− mice from CIA+ WT mice, and lastly, a third multiprotein signature (</span><em class="ms-rteFontSize-2">n</em><em class="ms-rteFontSize-2"> </em><span class="ms-rteFontSize-2">= 18) indicated that CD38</span><sup class="ms-rteFontSize-2">-</sup><sup class="ms-rteFontSize-2"><em>/</em></sup><sup class="ms-rteFontSize-2">-</sup><span class="ms-rteFontSize-2">and WT mice could be segregated in response to CFA treatment. Further analyses showed that the discriminative power to distinguish these groups was reached at protein species level and not at the protein level. Hence, the need to identify and quantify proteins at protein species level to better correlate proteome changes with disease processes. It is crucial for plasma proteomics at the low abundance protein species level to apply the ProteoMiner enrichment. All MS data have been deposited in the ProteomeXchange with identifiers PXD001788, PXD001799 and PXD002071 (http://proteomecentral.proteomexchange.org/dataset/PXD001788,</span><a href="http://proteomecentral/"><span lang="EN-US" class="ms-rteFontSize-2" style="text-decoration:underline;">http://proteomecentral</span></a><span class="ms-rteFontSize-2">. proteomexchange.org/dataset/PXD001799 and </span><a href="http://proteomecentral.proteomexchange.org/"><span lang="EN-US" class="ms-rteFontSize-2" style="text-decoration:underline;">http://proteomecentral.proteomexchange.org/</span></a><span class="ms-rteFontSize-2">dataset/PXD002071).</span><br></p> | <p><a href="https://www.ncbi.nlm.nih.gov/pubmed/26175002"><span class="ms-rteFontSize-2">Proteomics, 2015, 15: 3382-3393 doi: 10.1002/pmic.201400536. </span></a><br></p> | 162 | | |
