Tissue-specific requirements for FGF8 during early inner ear developmentTissue-specific requirements for FGF8 during early inner ear developmentDomínguez-Frutos E, Vendrell V, Alvarez Y, Zelarayan LC, López-Hernández I, Ros M, Schimmang T.2009-07-17T22:00:00Z<h2><span class="ms-rteThemeFontFace-1 ms-rteFontSize-2">​Abstract<br></span></h2><p><span class="ms-rteThemeFontFace-1 ms-rteFontSize-2"><span style="color:#2e2e2e;box-sizing:border-box;margin:0px;padding:0px;"><span style="box-sizing:border-box;margin:0px;padding:0px;"><br></span></span></span></p><p style="text-align:justify;"><span class="ms-rteThemeFontFace-1 ms-rteFontSize-2"><span style="color:#2e2e2e;box-sizing:border-box;margin:0px;padding:0px;"><span style="box-sizing:border-box;margin:0px;padding:0px;">Several members of the FGF gene family<span style="box-sizing:border-box;margin:0px;padding:0px;"><span style="box-sizing:border-box;margin:0px;padding:0px;"> have been shown to intervene from various tissue sources to direct otic placode induction and </span>otic vesicle formation. In this study we define the roles of </span></span>FGF8, found in different expression domains during this process, in mice and chickens. By conditional inactivation of </span><em style="color:#2e2e2e;box-sizing:border-box;margin:0px;padding:0px;">Fgf8</em><span style="color:#2e2e2e;"> in distinct tissue compartments we demonstrate that </span><em style="color:#2e2e2e;box-sizing:border-box;margin:0px;padding:0px;">Fgf8</em><span style="color:#2e2e2e;box-sizing:border-box;margin:0px;padding:0px;"> is required in the mesoderm <span style="box-sizing:border-box;margin:0px;padding:0px;">and endoderm during early inner ear development. In the chicken embryo, overexpression of </span></span><em style="color:#2e2e2e;box-sizing:border-box;margin:0px;padding:0px;">Fgf8</em><span style="color:#2e2e2e;"> from various tissue sources during otic specification leads to a loss of otic tissue. In contrast ectopic overexpression of </span><em style="color:#2e2e2e;box-sizing:border-box;margin:0px;padding:0px;">Fgf10</em><span style="color:#2e2e2e;box-sizing:border-box;margin:0px;padding:0px;">, a major player during murine otic induction, does not influence otic vesicle formation in chicken embryos but results in the formation of ectopic structures with a non-otic character. This study underlines the crucial role of a defined </span><em style="color:#2e2e2e;box-sizing:border-box;margin:0px;padding:0px;">Fgf8</em><span style="color:#2e2e2e;box-sizing:border-box;margin:0px;padding:0px;">expression pattern controlling inner ear formation in vertebrates.</span><br></span></p><p><span lang="EN-US"><a href="https://www.sciencedirect.com/science/article/pii/S0925477309013082">​Mech Dev. 2009 Jul 18.</a></span></p>210