Spectrophotometric Assays to Quantify the Activity of T4SS ATPasesSpectrophotometric Assays to Quantify the Activity of T4SS ATPasesCabezón E, Arechaga I.2019-10-04T22:00:00Z<h3>Abstract<br></h3><div> <br> </div><div>Biogenesis of T4SS apparatus and substrate transport require energy. Conjugative T4SS have three ATPases</div><div>that enable DNA processing and transport of the nucleoprotein complex to the recipient cell. In the</div><div>conjugative plasmid R388, these ATPases are named TrwB, TrwK, and TrwD. Here, three different</div><div>spectrophotometric assays to measure the enzymatic properties of these ATPases are described. The choice</div><div>of the assay will depend on the specific requirements of each enzyme.<br></div> <br><p><a href="https://link.springer.com/protocol/10.1007/978-1-4939-9877-7_10#citeas">​In: de la Cruz, F. (eds) Horizontal Gene Transfer. Methods in Molecular Biology, vol 2075. Humana, New York, NY., 2019</a><br></p>415