Abstract: The explosion in biological data generation challenges the available technologies and methodologies for data interrogation. Moreover, highly rich and complex datasets together with diverse linked data are difficult to explore when provided in flat files. Here we provide a way to filter and analyse in a systematic way a dataset with more than 18 thousand data points using Zegami, a solution for interactive data visualisation and exploration. The primary data we use are derived from a systematic analysis of 200 YFP gene traps reveals common discordance between mRNA and protein across the nervous system which is submitted elsewhere. This manual provides the raw image data together with annotations and associated data and explains how to use Zegami for exploring all these data types together by providing specific examples. We also provide the open source python code used to annotate the figures.

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