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Cytotoxicity analysis of oxazine 4-perchlorate fluorescence nerve potential clinical biomarker for guided surgery

Abstract: Biological tissue discrimination is relevant in guided surgery. Nerve identification is critical to avoid potentially severe collateral damage. Fluorescence imaging by oxazine 4-perchlorate (O4P) has been recently proposed. In this work, the cytotoxicity of O4P on U87 human-derived glioma cells has been investigated as a function of concentration and operating room irradiation modes. A custom-built optical irradiation device was employed for controlled optical dosimetry. DNA damage and O4P intracellular localization was also investigated by immunofluorescence and confocal microscopy. The results show that concentration below 100 µM can be considered safe. These results contribute to the assessment of the feasibility of O4P as a nerve biomarker.

 Autoría: Pampín-Suárez S., Arce-Diego J.L., Tapia O., Pérez-Campo F.M., Rodríguez-Rey J.C., Fanjul-Vélez F.,

 Fuente: Biomedical Optics Express, 2022, 13(1), 197-208

Editorial: The Optical Society

 Fecha de publicación: 01/01/2022

Nº de páginas: 12

Tipo de publicación: Artículo de Revista

 DOI: 10.1364/BOE.447773

ISSN: 2156-7085

 Proyecto español: PGC2018-101464-B-I00

Url de la publicación: https://doi.org/10.1364/BOE.447773