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Monitoring Bacterial Conjugation by Optical Microscopy

Abstract: Bacterial conjugation is the main mechanism for horizontal gene transfer, conferring plasticity to the genome repertoire. This process is also the major instrument for the dissemination of antibiotic resistance genes. Hence, gathering primary information of the mechanism underlying this genetic transaction is of a capital interest. By using fluorescent protein fusions to the ATPases that power conjugation, we have been able to track the localization of these proteins in the presence and absence of recipient cells. Moreover, we have found that more than one copy of the conjugative plasmid is transferred during mating. Altogether, these findings provide new insights into the mechanism of such an important gene transfer device.

 Fuente: Front Microbiol . 2021 Oct 4;12:750200

Editorial: Frontiers Research Foundation

 Año de publicación: 2021

Nº de páginas: 10

Tipo de publicación: Artículo de Revista

 DOI: 10.3389/fmicb.2021.750200

ISSN: 1664-302X

Proyecto español: BFU2016-78521-R

Url de la publicación: https://www.doi.org/10.3389/fmicb.2021.750200