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Abstract: cis-regulatory elements (CREs) orchestrate the spatiotemporal control of gene expression. The regulatory activity of CREs is typically assessed by reporter assays, in which CREs are studied outside their endogenous context. To circumvent this problem, we developed a CRISPR-Cas9 knockin approach to study CREs in a scar-free genomic context. Here, we describe the design, transfection, and screening protocol to insert CREs in mouse embryonic stem cells. Our strategy can provide important insights into the sufficiency of CREs for gene expression control.
Fuente: STAR Protocols, 2022, 3, 101492
Editorial: Elsevier
Año de publicación: 2022
Nº de páginas: 18
Tipo de publicación: Artículo de Revista
DOI: 10.1016/j.xpro.2022.101492
ISSN: 2666-1667
Proyecto español: MCIN/AEI /10.13039/501100011033
Url de la publicación: https://doi.org/10.1016/j.xpro.2022.101492
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