Buscar

Estamos realizando la búsqueda. Por favor, espere...

 Detalle_Publicacion

The Differential Organization of F-Actin Alters the Distribution of Organelles in Cultured When Compared to Native Chromaffin Cells

Abstract: Cultured bovine chromaffin cells have been used extensively as a neuroendocrine model to study regulated secretion. In order to extend such experimental findings to the physiological situation, it is necessary to study mayor cellular structures affecting secretion in cultured cells with their counterparts present in the adrenomedullary tissue. F-actin concentrates in a peripheral ring in cultured cells, as witnessed by phalloidin?rodhamine labeling, while extends throughout the cytoplasm in native cells. This result is also confirmed when studying the localization of ?-fodrin, a F-actin-associated protein. Furthermore, as a consequence of this redistribution of F-actin, we observed that chromaffin granules and mitochondria located into two different cortical and internal populations in cultured cells, whereas they are homogeneously distributed throughout the cytoplasm in the adrenomedullary tissue. Nevertheless, secretion from isolated cells and adrenal gland pieces is remarkably similar when measured by amperometry. Finally, we generate mathematical models to consider how the distribution of organelles affects the secretory kinetics of intact and cultured cells. Our results imply that we have to consider F-actin structural changes to interpret functional data obtained in cultured neuroendocrine cells.

 Autoría: Gimenez-Molina Y., Villanueva J., Nanclares C., Lopez-Font I., Viniegra S., Francés M.d.M., Gandia L., Gil A., Gutiérrez L.M.,

 Fuente: Frontiers in cellular neuroscience May 2017 Volume 11 Article 135

Editorial: Frontiers

 Fecha de publicación: 04/05/2017

Nº de páginas: 12

Tipo de publicación: Artículo de Revista

 DOI: 10.3389/fncel.2017.00135

ISSN: 1662-5102

Proyecto español: BFU2011-25095 ; BFU2015-63684-P

Autores/as

GIMENEZ-MOLINA, YOLANDA

VILLANUEVA, JOSÉ

NANCLARES, CARMEN

LOPEZ-FONT, INMACULADA

VINIEGRA, SALVADOR

FRANCÉS, MARIA DEL MAR

GANDIA, LUIS

GUTIÉRREZ, LUIS M.