Abstract: Introduction: Gordonia spp. infections are uncommon. However, a few clinical cases have been
reported in the literature, particularly those involving immunocompromised hosts. Advanced
microbiology diagnosis techniques, such as matrix-assisted laser desorption ionization-time of
flight MS (MALDI-TOF MS), have been recently introduced in clinical microbiology laboratories in
order to improve microbial identification, resulting in better patient management.
Case presentation: Here, we present a new clinical case of persistent wound infection caused
by Gordonia bronchialis in a 64-year-old woman after a mitral valve replacement, using two
MALDI-TOF-based systems for identifying this micro-organism.
Conclusion: Both MALDI-TOF systems were able to identify Gordonia spp.; thus, providing a
useful tool that overcomes the current limitations of phenotypic identification associated with this
micro-organism. Although the technique validation deserves additional verification, our study
provides guidance about MALDI-TOF as a fast and easy method for Gordonia spp.
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