Tuberculosis (TB) control strategies are focused mainly on prevention, early diagnosis,
compliance to treatment and contact tracing. The objectives of this study were to explore
the frequency and risk factors of recent transmission of clinical isolates of Mycobacterium
tuberculosis complex (MTBC) in Cantabria in Northern Spain from 2012 through 2013 and
to analyze their clonal complexity for better understanding of the transmission dynamics in
a moderate TB incidence setting.
DNA from 85 out of 87 isolates from bacteriologically confirmed cases of MTBC infection
were extracted directly from frozen stocks and genotyped using the mycobacterial interspersed
repetitive units-variable number tandem repeat (MIRU-VNTR) method. The MIRUVNTRplus
database tool was used to identify clusters and lineages and to build a neighbor
joining (NJ) phylogenetic tree. In addition, data were compared to the SITVIT2 database at
the Pasteur Institute of Guadeloupe.
The rate of recent transmission was calculated to 24%. Clustering was associated with
being Spanish-born. A high prevalence of isolates of the Euro-American lineage was found.
In addition, MIRU-VNTR profiles of the studied isolates corresponded to previously found
MIRU-VNTR types in other countries, including Spain, Belgium, Great Britain, USA, Croatia,
South Africa and The Netherlands. Six of the strains analyzed represented clonal variants. Conclusion
Transmission of MTBC is well controlled in Cantabria. The majority of TB patients were born
in Spain. The population structure of MTBC in Cantabria has a low diversity of major clonal
lineages with the Euro-American lineage predominating.
Fuente: PLoS ONE 2016 11(6): e0157266
Editorial: Public Library of Science
Año de publicación: 2016
Nº de páginas: 11
Tipo de publicación: Artículo de Revista
Consultar en UCrea Leer publicación
PÉREZ DEL MOLINO BERNAL, INMACULADA C.
PEDERSEN, MATHIAS K.
LUIS MARTINEZ MARTINEZ
FOLKVARDSEN, DORTE B.
JESUS AGÜERO BALBIN
RASMUSSEN, E. MICHAEL