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Abstract: Background: Rhodococcus jostii RHA1 and other actinobacteria accumulate triglycerides (TAG) under nutrient starvation.
This property has an important biotechnological potential in the production of sustainable oils.
Results: To gain insight into the metabolic pathways involved in TAG accumulation, we analysed the transcriptome
of R jostii RHA1 under nutrient-limiting conditions. We correlate these physiological conditions with significant
changes in cell physiology. The main consequence was a global switch from catabolic to anabolic pathways. Interestingly,
the Entner-Doudoroff (ED) pathway was upregulated in detriment of the glycolysis or pentose phosphate pathways.
ED induction was independent of the carbon source (either gluconate or glucose). Some of the diacylglycerol
acyltransferase genes involved in the last step of the Kennedy pathway were also upregulated. A common feature of
the promoter region of most upregulated genes was the presence of a consensus binding sequence for the cAMPdependent
Conclusion: This is the first experimental observation of an ED shift under nutrient starvation conditions. Knowledge
of this switch could help in the design of metabolomic approaches to optimize carbon derivation for single cell oil
Fuente: Microbial Cell Factories 2017 16: 35
Editorial: BioMed Central
Año de publicación: 2017
Nº de páginas: 12
Tipo de publicación: Artículo de Revista
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JUAN ANTONIO VILLA TORRECILLA
LANZA, VAL F.
FERNANDO DE LA CRUZ CALAHORRA
ÁLVAREZ, HÉCTOR M.
GABRIEL MONCALIAN MONTES