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Potassium-Ion-Selective Fluorescent Sensors To Detect Cereulide, the Emetic Toxin of B. cereus, in Food Samples and HeLa Cells

Abstract: We report the development of new chemical probes for cereulide, a toxic metabolite produced by specific strains of Bacillus cereus, through displacement of potassium cations from a preformed specific complex and a subsequent change in the fluorescence emission. For this purpose, we designed fluorescent probes for potassium cations that were suitable for displacement assays with cereulide from organic extracts. The fluorescence detection of natural cereulide in rice samples was achieved by using synthetic cereulide as a reference and a potassium fluorescent reporter, and this was found to be useful as a portable and fast method for the in situ detection of cereulide in food extracts. To study the fate of cereulide in live cells, we designed a procedure that was suitable for live-cell microscopy imaging of HeLa cells by comparing the cellular location of the potassium fluorogenic probe, which stained intracellular endolysosomes, in the absence and presence of cereulide; we concluded that in the presence of cereulide, the fluorescence of the probe was decreased because of complexation of the potassium ions by cereulide.

 Fuente: Chemistry Open 2017, 6, 562

Editorial: Wiley

 Año de publicación: 2017

Nº de páginas: 10

Tipo de publicación: Artículo de Revista

 DOI: 10.1002/open.201700057

ISSN: 2191-1363

Autores/as

GARCÍA CALVO, JOSÉ

IBEAS, SATURNINO

ANTÓN GARCÍA, EVA CLARA

TORROBA, TOMÁS

GONZÁLEZ AGUILAR, GERARDO

ANTUNES, WILSON

ELOISA GONZALEZ LAVADO