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Efficient differentiation of Corynebacterium striatum, Corynebacterium amycolatum and Corynebacterium xerosis clinical isolates by multiplex PCR using novel species-specific primers

Abstract: A multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226 bp, 434 bp and 106 bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR results were 100% in agreement with identifications achieved by 16S rRNA and rpoB gene sequencing and by VITEK-MS.

 Fuente: Journal of Microbiological Methods Volume 142, November 2017, Pages 33-35

Editorial: Elsevier

 Fecha de publicación: 01/11/2017

Nº de páginas: 13

Tipo de publicación: Artículo de Revista

DOI: 10.1016/j.mimet.2017.09.002

ISSN: 0167-7012,1872-8359

Autores/as

SANTOS, CAROLINA S.

RAMOS, JULIANA N.

VIEIRA, VERONICA V.

PINHEIRO, CARINA S.

MEYER, ROBERTO

ALCANTARA NEVES, NEUZA M.

RAMOS, ROMMEL T.

SILVA, ARTUR

HIRATA, RAPHAEL

FELICORI, LIZA

RUIZ DE ALEGRÍA PUIG, CARLOS

AZEVEDO, VASCO

MATTOS GUARALDI, ANA L.

PACHECO, LUIS G.C.