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Cardiolipin plays an essential role in the formation of intracellular membranes in Escherichia coli

Abstract: Mitochondria, chloroplasts and photosynthetic bacteria are characterized by the presence of complex and intricate membrane systems. In contrast, non-photosynthetic bacteria lack membrane structures within their cytoplasm. However, large scale over-production of some membrane proteins, such as the fumarate reductase, the mannitol permeaseMtlA, the glycerol acyl transferase PlsB, the chemotaxis receptor Tsr or the ATP synthase subunit b, can induce the proliferation of intra cellular membranes (ICMs) in the cytoplasm of Escherichia coli. These ICMs are particularly rich in cardiolipin (CL). Here, we have studied the effect of CL in the generation of these membranous structures.We have deleted the three genes (clsA, clsB and clsC) responsible of CL biosynthesis in E. coli and analysed the effect of these mutations by fluorescent and electron microscopy and by lipid mass spectrometry.We have found that CL is essential in the formation of non-lamellar structures in the cytoplasm of E. coli cells. These results could help to understand the structuration of membranes in E. coli and other membrane organelles, such as mitochondria and ER.

Otras publicaciones de la misma revista o congreso con autores/as de la Universidad de Cantabria

 Fuente: Biochimica et Biophysica Acta 1859 (2017) 1124-1132

Editorial: Elsevier

 Año de publicación: 2017

Nº de páginas: 9

Tipo de publicación: Artículo de Revista

 DOI: 10.1016/j.bbamem.2017.03.006

ISSN: 0005-2736,1879-2642

Autores/as

GERARDO CARRANZA FERRER

ANGIUS, FEDERICA

ILIOAIA, OANA

SOLGADI, AUDREY