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Abstract: Radiation and adjuvant endocrine therapy are nowadays considered
a standard treatment option after surgery in breast cancer. Melatonin exerts
oncostatic actions on human breast cancer cells. In the current study, we
investigated the effects of a combination of radiotherapy and melatonin on
human breast cancer cells. Melatonin (1 mM, 10 lM and 1 nM) significantly
inhibited the proliferation of MCF-7 cells. Radiation alone inhibited the
MCF-7 cell proliferation in a dose-dependent manner. Pretreatment of breast
cancer cells with melatonin 1 wk before radiation led to a significantly greater
decrease of MCF-7 cell proliferation compared with radiation alone.
Melatonin pretreatment before radiation also decreased G2-M phase arrest
compared with irradiation alone, with a higher percentage of cells in the
G0-G1 phase and a lower percentage of cells in S phase. Radiation alone
diminished RAD51 and DNA-protein kinase (PKcs) mRNA expression, two
main proteins involved in double-strand DNA break repair. Treatment with
melatonin for 7 days before radiation led to a significantly greater decrease in
RAD51 and DNAPKcs mRNA expression compared with radiation alone.
Our findings suggest that melatonin pretreatment before radiation sensitizes
breast cancer cells to the ionizing effects of radiation by decreasing cell
proliferation, inducing cell cycle arrest and downregulating proteins involved
in double-strand DNA break repair. These findings may have implications for
designing clinical trials using melatonin and radiotherapy.
Fuente: Journal of Pineal Research. 2015; 58:189–197
Editorial: Blackwell Publishing Ltd
Año de publicación: 2015
Nº de páginas: 9
Tipo de publicación: Artículo de Revista
CAROLINA ALONSO GONZALEZ
ALICIA VERONICA GONZALEZ CABEZA
CARLOS MANUEL MARTINEZ CAMPA
JOSE EZEQUIEL GOMEZ AROZAMENA
SAMUEL COS CORRAL