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 Detalle_Publicacion

Myc Stimulates Cell Cycle Progression Through the Activation of Cdk1 and Phosphorylation of p27

Abstract: Cell cycle stimulation is a major transforming mechanism of Myc oncoprotein. This is achieved through at least three concomitant mechanisms: upregulation of cyclins and Cdks, downregulation of the Cdk inhibitors p15 and p21 and the degradation of p27. The Myc-p27 antagonism has been shown to be relevant in human cancer. To be degraded, p27 must be phosphorylated at Thr-187 to be recognized by Skp2, a component of the ubiquitination complex. We previously described that Myc induces Skp2 expression. Here we show that not only Cdk2 but Cdk1 phosphorylates p27 at the Thr-187. Moreover, Myc induced p27 degradation in murine fibroblasts through Cdk1 activation, which was achieved by Myc-dependent cyclin A and B induction. In the absence of Cdk2, p27 phosphorylation at Thr-187 was mainly carried out by cyclin A2-Cdk1 and cyclin B1-Cdk1. We also show that Cdk1 inhibition was enough for the synthetic lethal interaction with Myc. This result is relevant because Cdk1 is the only Cdk strictly required for cell cycle and the reported synthetic lethal interaction between Cdk1 and Myc.

Otras publicaciones de la misma revista o congreso con autores/as de la Universidad de Cantabria

 Fuente: Sci Rep , 9 (1), 18693

Editorial: Nature Publishing Group

 Año de publicación: 2019

Nº de páginas: 27

Tipo de publicación: Artículo de Revista

 DOI: 10.1038/s41598-019-54917-1

ISSN: 2045-2322

Proyecto español: SAF2017-88026-R

Url de la publicación: https://doi.org/10.1038/s41598-019-54917-1

Autores/as

LUCIA GARCIA GUTIERREZ

GABRIEL BRETONES SANCHEZ

SYMONDS, CATHERINE

ACOSTA, JUAN C.

FERNÁNDEZ, ADRIÁN

ALONSO, LETICIA

SICINSKI, PIOTR

BARBACID, MARIANO

SANTAMARÍA, DAVID