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PAUF/ZG16B Promotes Colorectal Cancer Progression Through Alterations of the Mitotic Functions and the Wnt/B-catenin Pathway

Abstract: Pancreatic adenocarcinoma upregulated factor (PAUF), also known as ZG16B, was previously found in the secretome of metastatic colorectal cancer cells. Here, we demonstrated the presence of PAUF at the intracellular level and its multiple effects on cancer progression. An initial decline of PAUF expression was observed at early stages of colorectal cancer followed by an increase at the metastatic site. PAUF was located at different cellular compartments: membrane-associated vesicles, endosomes, microtubule-associated vesicles, cell growth cones and the cell nucleus. PAUF loss in two colorectal cancer cell lines caused severe alterations in the cell phenotype and cell cycle, including tetraploidy, extensive genomic alterations, micronuclei and increased apoptosis. An exhaustive analysis of the PAUF interactome using different proteomic approaches revealed the presence of multiple components of the cell cycle, mitotic checkpoint, Wnt pathway and intracellular transport. Among the interacting proteins we found ZW10, a moonlighting protein with a dual function in membrane trafficking and mitosis. In addition, PAUF silencing was associated to APC loss and increased ?-catenin nuclear expression. Altogether, our results suggest that PAUF depletion increases aneuploidy, promotes apoptosis and activates the Wnt/?-catenin pathway in colorectal cancer cells facilitating cancer progression. In summary, PAUF behaves as a multifunctional protein, with different roles in cancer progression according to the extra- or intracellular expression, suggesting a therapeutic value for colorectal cancer.

 Fuente: Carcinogenesis . 2020 Apr 22;41(2):203-213

Editorial: Oxford University Press

 Año de publicación: 2020

Nº de páginas: 11

Tipo de publicación: Artículo de Revista

 DOI: 10.1093/carcin/bgz093

ISSN: 0143-3334,1460-2180

Proyecto español: BIO2015-66489-R

Url de la publicación: https://www.doi.org/10.1093/carcin/bgz093

Autores/as

ESCUDERO-PANIAGUA, BEATRIZ

BARTOLOMÉ, RUBÉN A.

RODRÍGUEZ, SANDRA

RÍOS, VIVIAN DE LOS

PINTADO, LAURA

JAÉN, MARTA

MIGUEL ANGEL LAFARGA COSCOJUELA

FERNÁNDEZ-ACEÑERO, MARIA JESÚS

CASAL, J. IGNACIO