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A custom-made functionalization method to control the biological identity of nanomaterials

Abstract: Here we propose a one-step strategy to endow nanomaterials with a custom-designed bio-identity. This study designs a universal ?nanomaterial binding domain? that can be genetically attached to any protein ensuring precise and spontaneous protein orientation. We demonstrate how, despite the simplicity of the method, the bioconjugation achieved: (i) is highly efficient, even in the presence of competing proteins, (ii) is stable at extreme physiological conditions (pH ranges 5.2-9.0; NaCl concentrations 0-1?M); (iii) prevents unwanted protein biofouling days after incubation in biologically-relevant conditions; and finally, (iv) avoids nanoparticle interaction with promiscuous unspecific receptors. In summary, this protein biocoating technique, applicable to a wide array of nano-designs, integrates material science and molecular biology procedures to create hybrid nanodevices with well-defined surfaces and predictable biological behaviors, opening a chapter in precision nanodiagnostics, nanosensing or nanotherapeutic applications

Otras publicaciones de la misma revista o congreso con autores/as de la Universidad de Cantabria

 Fuente: Nanomedicine: Nanotechnology, Biology, and Medicine 29 (2020) 102268

Editorial: Future Medicine Ltd.

 Año de publicación: 2020

Nº de páginas: 9

Tipo de publicación: Artículo de Revista

DOI: 10.1016/j.nano.2020.102268

ISSN: 1743-5889,1748-6963

Autores/as

ESPERANZA PADIN GONZALEZ

ELENA NAVARRO PALOMARES

LOURDES MARIA VALDIVIA FERNANDEZ

NEREA ITURRIOZ RODRIGUEZ

CORREA, MIGUEL ANGEL