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 Detalle_Publicacion

A conditional piggyBac transposition system for genetic screening in mice identifies oncogenic networks in pancreatic cancer

Abstract: Here we describe a conditional piggyBac transposition system in mice and report the discovery of large sets of new cancer genes through a pancreatic insertional mutagenesis screen. We identify Foxp1 as an oncogenic transcription factor that drives pancreatic cancer invasion and spread in a mouse model and correlates with lymph node metastasis in human patients with pancreatic cancer. The propensity of piggyBac for open chromatin also enabled genome-wide screening for cancer-relevant noncoding DNA, which pinpointed a Cdkn2a cis-regulatory region. Histologically, we observed different tumor subentities and discovered associated genetic events, including Fign insertions in hepatoid pancreatic cancer. Our studies demonstrate the power of genetic screening to discover cancer drivers that are difficult to identify by other approaches to cancer genome analysis, such as downstream targets of commonly mutated human cancer genes. These piggyBac resources are universally applicable in any tissue context and provide unique experimental access to the genetic complexity of cancer

 Fuente: Nat Genet. 2015 Jan;47(1):47-56

Editorial: Nature Publishing Group

 Año de publicación: 2014

Nº de páginas: 10

Tipo de publicación: Artículo de Revista

 DOI: 10.1038/ng.3164

ISSN: 1061-4036,1546-1718

Autores/as

RAD, ROLAND

RAD, LENA

WANG, WEI

STRONG, ALEXANDER

PONSTINGL, HANNES

BRONNER, IRAAD F

MAYHO, M

STEIGER, K

WEBER, JULIA

HIEBER, MAREN

VELTKAMP, C

ESER, STEFAN

GEUMANN, ULF

ÓLLINGER, RUPERT

ZUKOWSKA, MAGDALENA

BARENBOIM, MAXIM

MARESCH, ROMAN

CADIÑANOS, JUAN

FRIEDRICH, MATHIAS

CONSTANTINO CASAS, FERNANDO

SARVER, AARON

HOEVE, JELLE TEN

PROSSER, HAYDN

SEIDLER, BARBARA

BAUER, JUDITH

HEIKENWÄLDER, MATHIAS

METZAKOPIAN, EMMANOUIL

KRUG, ANNE

EHMER, URSULA

SCHNEIDER, GÜNTER

KNÖSEL, THOMAS

RÜMMELE, PETRA

AUST, DANIELA

GRÜTZMANN, ROBERT

PILARSKY, CHRISTIAN

NING, ZEMI

WESSELS, LODEWYK

SCHMID, ROLAND

QUAIL, MICHAEL A

VASSILIOU, GEORGE

ESPOSITO, IRENE

LIU, PENTAOU

SAUR, DIETER

BRADLEY, ALLAN