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CryoSIM: super-resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultrastructural imaging

Abstract: Rapid cryopreservation of biological specimens is the gold standard for visualizing cellular structures in their true structural context. However, current commercial cryo-fluorescence microscopes are limited to low resolutions. To fill this gap, we have developed cryoSIM, a microscope for 3D super-resolution fluorescence cryo-imaging for correlation with cryo-electron microscopy or cryo-soft X-ray tomography. We provide the full instructions for replicating the instrument mostly from off-the-shelf components and accessible, user-friendly, open-source Python control software. Therefore, cryoSIM democratizes the ability to detect molecules using super-resolution fluorescence imaging of cryopreserved specimens for correlation with their cellular ultrastructure.

 Fuente: Optica. 2020 (7): 802-812

Editorial: Optical Society of America

 Año de publicación: 2020

Nº de páginas: 11

Tipo de publicación: Artículo de Revista

 DOI: 10.1364/OPTICA.393203

ISSN: 2334-2536

Url de la publicación: https://doi.org/10.1364/OPTICA.393203

Autores/as

PHILLIPS, MICHAEL A.

HARKIOLAKI, MARIA

SUSANO PINTO, DAVID MIGUEL

PARTON, RICHARD M.

GARCÍA MORENO, MANUEL

KOUNATIDIS, ILIAS

SEDAT, JOHN W.

STUART, DAVID I.

CASTELLO, ALFREDO

BOOTH, MARTIN J.

DAVIS, ILAN

DOBBIE, IAN M.