Abstract: Corn is one of the main food products in Mexico. The elaboration of corn-derived products generates wastewater with a high organic load (nejayote). Anaerobic digestion is an indicated treatment for wastewater with high organic loads. The results of this study show that the application of microaeration in the hydrolysis-fermentative reactor increased the percentage of volatile fatty acids (VFA) available in the medium by 62%. The addition of a conductive material, such as granulated activated carbon (GAC), promotes DIET (Direct interspecies electrons transfer) in the methanogenic UASB reactor increasing the methane yield by 55%. Likewise, a great diversity of exoelectrogenic bacteria, with the ability to donate electrons DIET mechanisms, were developed in the GAC biofilm, though interestingly, Peptoclostridium and Clostridium (17.3% and 12.75%, respectively) were detected with a great abundance in the GAC biofilm. Peptoclostridium has not been previously reported as a participant in DIET process.

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