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 Detalle_Publicacion

The chaperonin CCT controls T cell receptor-driven 3D configuration of centrioles

Abstract: T lymphocyte activation requires the formation of immune synapses (IS) with antigen-presenting cells. The dynamics of membrane receptors, signaling scaffolds, microfilaments, and microtubules at the IS determine the potency of T cell activation and subsequent immune response. Here, we show that the cytosolic chaperonin CCT (chaperonin-containing TCP1) controls the changes in reciprocal orientation of the centrioles and polarization of the tubulin dynamics induced by T cell receptor in T lymphocytes forming an IS. CCT also controls the mitochondrial ultrastructure and the metabolic status of T cells, regulating the de novo synthesis of tubulin as well as posttranslational modifications (poly-glutamylation, acetylation, ?1 and ?2) of ??-tubulin heterodimers, fine-tuning tubulin dynamics. These changes ultimately determine the function and organization of the centrioles, as shown by three-dimensional reconstruction of resting and stimulated primary T cells using cryo-soft x-ray tomography. Through this mechanism, CCT governs T cell activation and polarity.

 Fuente: Sci Adv . 2020 Dec 2;6(49):eabb7242.

Editorial: American Association for the Advancement of Science

 Año de publicación: 2020

Nº de páginas: 18

Tipo de publicación: Artículo de Revista

 DOI: 10.1126/sciadv.abb7242.

ISSN: 2375-2548

Proyecto español: SAF2017-82886-R ; PID2019-105872GB-I00 ; BFU2016-75984-P ; BIO2015-67580-P ; PGC2018-097019-B-I00 ; BFU2012-37163 ; BFU2015-68726-P

Url de la publicación: https://doi.org/10.1126/sciadv.abb7242

Autores/as

MARTIN COFRECES, N. B.

CHICHÓN, F. J.

CALVO, E.

TORRALBA, D.

BUSTOS MORÁN, E.

DOSIL, S. G.

ROJAS GÓMEZ, A.

BONZON KULICHENKO, E.

LÓPEZ, J. A.

OTÓN, J.

SORRENTINO, A.

VERNOS, I.

VÁZQUEZ, J.

VALPUESTA, J. M.

SÁNCHEZ MADRID, F.