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Accurate determination of key surface properties that determine the efficient separation of bovine milk BSA and LF proteins

Abstract: The aim of this work is to accurately measure fundamental surface properties, i.e., zeta potential, isoelectric point and protein size that determine the optimal separation conditions of Bovine serum albumin and lactoferrin, two high added value food proteins whose similarity in weight makes their separation a scientific and technical challenge. The systematic study of these proteins’ surface properties was performed under different conditions: (i) 3.0 < pH < 10.0, (ii) electrolyte type: KCl, NaCl and CaCl2 and concentration (0.01–0.1 M KCl) and (iii) protein concentration in the range of 0.04–4.0 g L-1 for BSA and 0.01–1.0 g L-1 for LF with the objective of establishing the optimal separation conditions. Finally, the comparison of the experimental and theoretically calculated values revealed significant deviations under specific conditions, highlighting the simplicity of the theoretical assumptions and leading to the conclusion that the use of experimental surface properties is still needed for the correct design of food protein separation processes.

Otras publicaciones de la misma revista o congreso con autores/as de la Universidad de Cantabria

 Autoría: Valiño V., San Román M., Ibáñez R., Benito J., Escudero I., Ortiz I.,

 Fuente: Separation and Purification Technology, 2014, 135, 145–157

Editorial: Elsevier

 Fecha de publicación: 01/10/2014

Nº de páginas: 13

Tipo de publicación: Artículo de Revista

 DOI: 10.1016/j.seppur.2014.07.051

ISSN: 1383-5866,1873-3794

Proyecto español: CTQ2011-25262 ; CTM2011-23912 ; CTQ2012-31639

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