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Standardization procedure for flow cytometry data harmonization in prospective multicenter studies

Abstract: One of the most challenging objective for clinical cytometry in prospective multicenter immunomonitoring trials is to compare frequencies, absolute numbers of leukocyte populations and further the mean fluorescence intensities of cell markers, especially when the data are generated from different instruments. Here, we describe an innovative standardization workflow to compare all data to carry out any large-scale, prospective multicentric flow cytometry analysis whatever the duration, the number or type of instruments required for the realization of such projects

Otras publicaciones de la misma revista o congreso con autores/as de la Universidad de Cantabria

Discovery of genes and proteins possibly regulating mean wool fibre diameter using cDNA microarray and proteomic approaches - Artículo de Revista
A predominant involvement of the triple seropositive patients and others with rheumatoid factor in the association of smoking with rheumatoid arthritis - Artículo de Revista
A cross-disease meta-GWAS identifies four new susceptibility loci shared between systemic sclerosis and Crohn's disease - Artículo de Revista
New insights for understanding spatial patterning and formation processes of the Neanderthal occupation in the Amalda I cave (Gipuzkoa, Spain) - Artículo de Revista
Omentin: a biomarker of cardiovascular risk in individuals with axial spondyloarthritis - Artículo de Revista
Influence of MUC5B gene on antisynthetase syndrome - Artículo de Revista
Future behavior of wind wave extremes due to climate change - Artículo de Revista
Micro-contextual identification of archaeological lipid biomarkers using resin-impregnated sediment slabs - Artículo de Revista
Hybrid Raman-erbium random fiber laser with a half open cavity assisted by artificially controlled backscattering fiber reflectors - Artículo de Revista
Analysis of Ani s 7 and Ani s 1 allergens as biomarkers of sensitization and allergy severity in human anisakiasis - Artículo de Revista
The probabilistic backbone of data-driven complex networks: an example in climate - Artículo de Revista
Microdrilled tapers to enhance optical fiber lasers for sensing - Artículo de Revista
Role of MUC1 rs4072037 polymorphism and serum KL-6 levels in patients with antisynthetase syndrome - Artículo de Revista
First modern human settlement recorded in the Iberian hinterland occurred during Heinrich Stadial 2 within harsh environmental conditions - Artículo de Revista
Feasibility of large-scale population testing for SARS-CoV-2 detection by self-testing at home - Artículo de Revista
Genomics of high molecular weight plasmids isolated from an on-farm biopurification system - Artículo de Revista
BAFF, APRIL and BAFFR on the pathogenesis of immunoglobulin-A vasculitis - Artículo de Revista
Role of the IL33 and IL1RL1 pathway in the pathogenesis of immunoglobulin-A vasculitis - Artículo de Revista
Adequacy of early-stage breast cancer systemic adjuvant treatment to Saint Gallen-2013 statement: the MCC-Spain study - Artículo de Revista
Sleep duration and napping in relation to colorectal and gastric cancer in the MCC-Spain study - Artículo de Revista
Crystal-field mediated electronic transitions of EuS up to 35 GPa - Artículo de Revista
A new approach to radon temporal correction factor based on active environmental monitoring devices - Artículo de Revista
PIK3CA mutation enrichment and quantitation from blood and tissue - Artículo de Revista
A simple and robust real-time qPCR method for the detection of PIK3CA mutations - Artículo de Revista
Evaluation of endothelial function and subclinical atherosclerosis in patients with HIV infection - Artículo de Revista
Molecular characterization of multidrug resistant Enterobacterales strains isolated from liver and kidney transplant recipients in Spain - Artículo de Revista
Temporary Cable Force MonitoringTechniques during Bridge Construction-Phase: The Tajo River Viaduct Experience - Artículo de Revista
Magnetic order and disorder environments in superantiferromagnetic NdCu2 nanoparticles - Artículo de Revista
Human forager response to abrupt climate change at 8.2 ka on the Atlantic coast of Europe - Artículo de Revista
Cost of stay and characteristics of patients with stroke and delayed discharge for non-clinical reasons - Artículo de Revista
Quasi-dark resonances with antiferromagnetic order in silicon metasurfaces - Artículo de Revista
Influence of the properties of different graphene-based nanomaterials dispersed in polycaprolactone membranes on astrocytic differentiation - Artículo de Revista
Skeletal Anomalies in The Neandertal Family of El Sidrón (Spain) Support A Role of Inbreeding in Neandertal Extinction - Artículo de Revista
Interleukin 1 receptor antagonist relation to cardiovascular disease risk in patients with rheumatoid arthritis - Artículo de Revista
A high birefringence liquid crystal for lenses with large aperture - Artículo de Revista
A paradigm shift in the quantification of wave energy attenuation due to saltmarshes based on their standing biomass - Artículo de Revista
Copper(II) invigorated EHU-30 for continuous electroreduction of CO2 into value-added chemicals - Artículo de Revista
Associations between serum mitokine levels and outcomes in stable COPD: an observational prospective study - Artículo de Revista
Calcineurin inhibitors' impact on cardiovascular and renal function, a descriptive study in lung transplant recipients from the North of Spain - Artículo de Revista
Coastal engineering infrastructure impacts Blue Carbon habitats distribution and ecosystem functions - Artículo de Revista
Sleep and breast and prostate cancer risk in the MCC-Spain study - Artículo de Revista
Phase stability and dense polymorph of the BaCa(CO3)2 barytocalcite carbonate - Artículo de Revista
The arrival of millets to the Atlantic coast of northern Iberia - Artículo de Revista
Temporal and spatial variability of prehistoric aquatic resource procurement: a case study from Mesolithic Northern Iberia - Artículo de Revista
Janus-Nanojet as an efficient asymmetric photothermal source - Artículo de Revista
A Unified Framework for Reservoir Computing and Extreme Learning Machines based on a Single Time-delayed Neuron - Artículo de Revista
Small dielectric spheres with high refractive index as new multifunctional elements for optical devices - Artículo de Revista
Expression of osteoprotegerin and its ligands, RANKL and TRAIL, in rheumatoid arthritis - Artículo de Revista
DNA damage precedes apoptosis during the regression of the interdigital tissue in vertebrate embryos - Artículo de Revista
The novel adipokine progranulin counteracts IL-1 and TLR4-driven inflammatory response in human and murine chondrocytes via TNFR1 - Artículo de Revista
A global classification of coastal flood hazard climates associated with large-scale oceanographic forcing - Artículo de Revista
Influence of elevated-CRP level-related polymorphisms in non-rheumatic caucasians on the risk of subclinical atherosclerosis and cardiovascular disease in rheumatoid arthritis - Artículo de Revista
Influence of coronary artery disease and subclinical atherosclerosis related polymorphisms on the risk of atherosclerosis in rheumatoid arthritis - Artículo de Revista
Risk model for colorectal cancer in spanish population using environmental and genetic factors: results from the MCC-Spain study - Artículo de Revista
A genome-wide association study suggests the HLA class II region as the major susceptibility locus for IgA vasculitis - Artículo de Revista
Protein tyrosine phosphatase non-receptor 22 and C-Src tyrosine kinase genes are down-regulated in patients with rheumatoid arthritis - Artículo de Revista
Risk model for prostate cancer using environmental and genetic factors in the spanish multi-case-control (MCC) study. - Artículo de Revista
Analysis of the common genetic component of large-vessel vasculitides through a meta- Immunochip strategy - Artículo de Revista
Occurrence of Corynebacterium striatum as an emerging antibiotic-resistant nosocomial pathogen in a Tunisian hospital - Artículo de Revista
Human neutrophils phagocytose and kill Acinetobacter baumanii and A. pittii - Artículo de Revista
Analysis of the R1b-DF27 haplogroup shows that a large fraction of Iberian Y-chromosome lineages originated recently in situ - Artículo de Revista
Whole genome sequencing, molecular typing and in vivo virulence of OXA-48-producing Escherichia coli isolates including ST131 H30-Rx, H22 and H41 subclones - Artículo de Revista
SNPs in bone-related miRNAs are associated with the osteoporotic phenotype - Artículo de Revista
Light guiding and switching using eccentric core-shell geometries - Artículo de Revista
Structural and magnetic properties of multi-core nanoparticles analysed using a generalised numerical inversion method - Artículo de Revista
Association of circulating calprotectin with lipid profile in axial spondyloarthritis - Artículo de Revista
Possible role of chondroitin sulphate and glucosamine for primary prevention of colorectal cancer. Results from the MCC-Spain study - Artículo de Revista
Validating a breast cancer score in spanish women. The MCC-Spain study - Artículo de Revista
Vitamin D exposure and risk of breast cancer: a meta-analysis - Artículo de Revista
Accumulation of poly(A) RNA in nuclear granules enriched in Sam68 in motor neurons from the SMNA7 mouse model of SMA - Artículo de Revista
On the scattering directionality of a dielectric particle dimer of High Refractive Index - Artículo de Revista
Changing environments during the Middle-Upper Palaeolithic transition in the eastern Cantabrian Region (Spain): direct evidence from stable isotope studies on ungulate bones - Artículo de Revista
High sensitization efficiency and energy transfer routes for population inversion at low pump intensity in Er organic complexes for IR amplification - Artículo de Revista
Sensitization, energy transfer and infra-red emission decay modulation in Yb3+-doped NaYF4 nanoparticles with visible light through a perfluoroanthraquinone chromophore - Artículo de Revista
Association of a rare variant of the TNFSF13B gene with susceptibility to Rheumatoid Arthritis and Systemic Lupus Erythematosus - Artículo de Revista
Environmental factors linked to depression vulnerability are associated with altered cerebellar resting-state synchronization - Artículo de Revista
Numerical analysis of bead magnetophoresis from flowing blood in a continuous-flow microchannel: implications to the bead-fluid interactions - Artículo de Revista
Pressure-induced spin transition and site-selective metallization in CoCl2 - Artículo de Revista
Bionanocomposites based on chitosan intercalation in designed swelling high-charged micas - Artículo de Revista
Suppression of BCL6 Function by HDAC Inhibitor Mediated Acetylation and Chromatin Modification Enhances BET Inhibitor Effects in B-cell Lymphoma Cells - Artículo de Revista
Experimental modelling of cardiac pressure overload hypertrophy: Modified technique for precise, reproducible, safe and easy aortic arch banding-debanding in mice - Artículo de Revista
Myc Stimulates Cell Cycle Progression Through the Activation of Cdk1 and Phosphorylation of p27 - Artículo de Revista
Antibody responses to flagellin C and Streptococcus gallolyticus pilus proteins in colorectal cancer - Artículo de Revista
A join point regression analysis of trends in mortality due to osteoporosis in Spain - Artículo de Revista
Mendelian randomization analysis rules out disylipidaemia as colorectal cancer cause - Artículo de Revista
Cellular and animal models of skin alterations in the autism-related ADNP syndrome - Artículo de Revista
Post-tilleyite, a dense calcium silicate-carbonate phase - Artículo de Revista
Brewster quasi bound states in the continuum in all-dielectric metasurfaces from single magnetic-dipole resonance meta-atoms - Artículo de Revista
Adaptability, resilience and environmental buffering in European Refugia during the Late Pleistocene: Insights from La Riera Cave (Asturias, Cantabria, Spain - Artículo de Revista
TGFB2-induced Senescence During Early Inner Ear Development - Artículo de Revista
Identification of Tipifarnib Sensitivity Biomarkers in T-cell Acute Lymphoblastic Leukemia and T-cell Lymphoma - Artículo de Revista
Positive-negative tunable liquid crystal lenses based on a microstructured transmission line - Artículo de Revista
Survival Impact of Primary Tumor Resection in De Novo Metastatic Breast Cancer Patients (GEICAM/El Alamo Registry) - Artículo de Revista
The Global flood protection Benefits of Mangroves - Artículo de Revista
Biofilm formation by multidrug resistant Enterobacteriaceae strains isolated from solid organ transplant recipients - Artículo de Revista
Multifunctional light beam control device by stimuli-responsive liquid crystal micro-grating structures - Artículo de Revista
Magnetosomes could be protective shields against metal stress in magnetotactic bacteria - Artículo de Revista
Nusinersen ameliorates motor function and prevents motoneuron Cajal body disassembly and abnormal poly(A) RNA distribution in a SMA mouse model - Artículo de Revista
Nanomechanics of graphene oxide-bacteriophage based self-assembled porous composites - Artículo de Revista
Blind testing of shoreline evolution models - Artículo de Revista
Climate-induced variability in South Atlantic wave direction over the past three millennia. - Artículo de Revista
Liquid level sensor based on dynamic Fabry-Perot interferometers in processed capillary fiber - Artículo de Revista
Usefulness of melatonin as complementary to chemotherapeutic agents at different stages of the angiogenic process - Artículo de Revista

Fuente: Scientific Reports 10, 11567 (2020).

Editorial: Nature Publishing Group

Año de publicación: 2020

Nº de páginas: 8

Tipo de publicación: Artículo de Revista

DOI: 10.1038/s41598-020-68468-3

ISSN: 2045-2322

Url de la publicación: https://doi.org/10.1038/s41598-020-68468-3

Consultar en UCrea Leer publicación

Autores/as

LUCAS LE LANN

PIERRE-EMMANUEL JOUVE

MARTA ALARCÓN-RIQUELME

CHRISTOPHE JAMIN

JACQUES-OLIVIER PERS

RICARDO BLANCO ALONSO

MIGUEL ANGEL GONZALEZ-GAY MANTECON

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