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Rapid, scalable assessment of SARS-CoV-2 cellular immunity by whole-blood PCR

Abstract: Fast, high-throughput methods for measuring the level and duration of protective immune responses to SARS-CoV-2 are needed to anticipate the risk of breakthrough infections. Here we report the development of two quantitative PCR assays for SARS-CoV-2-specific T cell activation. The assays are rapid, internally normalized and probe-based: qTACT requires RNA extraction and dqTACT avoids sample preparation steps. Both assays rely on the quantification of CXCL10 messenger RNA, a chemokine whose expression is strongly correlated with activation of antigen-specific T cells. On restimulation of whole-blood cells with SARS-CoV-2 viral antigens, viral-specific T cells secrete IFN-?, which stimulates monocytes to produce CXCL10. CXCL10 mRNA can thus serve as a proxy to quantify cellular immunity. Our assays may allow large-scale monitoring of the magnitude and duration of functional T cell immunity to SARS-CoV-2, thus helping to prioritize revaccination strategies in vulnerable populations.

 Fuente: Nature Biotechnology 2022 Nov;40(11):1680-1689

 Editorial: Springer Nature

 Año de publicación: 2022

 Nº de páginas: 10

 Tipo de publicación: Artículo de Revista

 DOI: 10.1038/s41587-022-01347-6

 ISSN: 1087-0156,1546-1696

 Url de la publicación: https://doi.org/10.1038/s41587-022-01347-6

Autoría

SCHWARZ, MEGAN

TORRE, DENIS

LOZANO-OJALVO, DANIEL

TAN, ANTHONY T.

TABAGLIO, TOMMASO

MZOUGHI, SLIM

SÁNCHEZ-TEJUELO, RODRIGO

LE BERT, NINA

MING ER LIM, JOEY

HATEM, SANDRA

TUBALLES, KEVIN

CÁMARA, CARMEN

LÓPEZ-GRANADOS, EDUARDO

PAZ-ARTAL, ESTELA

CORREA-ROCHA, RAFAEL

ORTIZ, ALBERTO

PORTOLES, JOSÉ

CERVERA, ISABEL

GONZÁLEZ-PÉREZ, MARÍA