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Nanoparticle-mediated selective Sfrp-1 silencing enhances bone density in osteoporotic mice

Abstract: Osteoporosis (OP) is characterized by a loss in bone mass and mineral density. The stimulation of the canonical Wnt/B-catenin pathway has been reported to promote bone formation, this pathway is controlled by several regulators as secreted frizzled-related protein-1 (Sfrp-1), antagonist of the pathway. Thus, Sfrp-1 silencing therapies could be suitable for enhancing bone growth. However, the systemic stimulation of Wnt/B-catenin has been correlated with side effects. This work hypothesizes the administration of lipid-polymer NPs (LPNPs) functionalized with a MSC specific aptamer (Apt) and carrying a SFRP1 silencing GapmeR, could favor bone formation in OP with minimal undesired effects. Suitable SFRP1 GapmeR-loaded Apt-LPNPs (Apt-LPNPs-SFRP1) were administered in osteoporotic mice and their biodistribution, toxicity and bone induction capacity were evaluated. The aptamer functionalization of the NPs modified their biodistribution profile showing a four-fold increase in the bone accumulation and a ten-fold decrease in the hepatic accumulation compared to naked LPNPs. Moreover, the histological evaluation revealed evident changes in bone structure observing a more compact trabecular bone and a cortical bone thickness increase in the Apt-LPNPs-SFRP1 treated mice with no toxic effects. Therefore, these LPNPs showed suitable properties and biodistribution profiles leading to an enhancement on the bone density of osteoporotic mice.

Otras publicaciones de la misma revista o congreso con autores/as de la Universidad de Cantabria

 Autoría: García-García P., Reyes R., García-Sánchez D., Pérez-Campo F.M., Rodríguez-Rey J.C., Évora C., Díaz-Rodríguez P., Delgado A.,

 Fuente: J Nanobiotechnology . 2022 Oct 29;20(1):462

Editorial: BioMed Central - Springer Nature

 Año de publicación: 2022

Nº de páginas: 19

Tipo de publicación: Artículo de Revista

 DOI: 10.1186/s12951-022-01674-5

ISSN: 1477-3155

 Proyecto español: RTI2018-097324-B-100

Url de la publicación: https://doi.org/10.1186/s12951-022-01674-5

Autoría

GARCÍA-GARCÍA, PATRICIA

REYES, RICARDO

DANIEL GARCIA SANCHEZ

ÉVORA, CARMEN

DÍAZ-RODRÍGUEZ, PATRICIA

DELGADO, ARACELI