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Targeted bacterial conjugation mediated by synthetic cell-to-cell adhesions

Abstract: Genetic interventions on microbiomes, for clinical or biotechnological purposes, remain challenging. Conjugation-based delivery of genetic cargo is still unspecific and limited by low conjugation rates. Here we report an approach to overcome these problems, based on a synthetic bacterial adhesion system. Mating assemblers consist on a synthetic adhesion formed by the expression on the surface of donor and target cells of specific nanobodies (Nb) and their cognate antigen (Ag). The Nb?Ag bridge increased 1?3 logs transfer of a variety of plasmids, especially in liquid media, confirming that cell-cell docking is a main determinant limiting mating efficiency. Synthetic cell-to-cell adhesion allows efficient conjugation to targeted recipients, enhancing delivery of desired genes to a predefined subset of prey species, or even specific pathogenic strains such as enterohemorrhagic Escherichia coli (EHEC), within a bacterial community. The synthetic conjugation enhancer presented here optimizes plasmid delivery by selecting the target hosts with high selectivity.

 Fuente: Nucleic Acids Research, 2022, 50(22), 12938-12950

Editorial: Oxford University Press

 Año de publicación: 2022

Nº de páginas: 13

Tipo de publicación: Artículo de Revista

 DOI: 10.1093/nar/gkac1164

ISSN: 0305-1048,1362-4962

 Proyecto español: BIO2017-89081-R

Url de la publicación: https://doi.org/10.1093/nar/gkac1164