Buscar

Estamos realizando la búsqueda. Por favor, espere...

 Detalle_Publicacion

A flow cytometry-based neutralization assay for simultaneous evaluation of blocking antibodies against SARS-CoV-2 variants

Abstract: Vaccines against SARS-CoV-2 have alleviated infection rates, hospitalization and deaths associated with COVID-19. In order to monitor humoral immunity, several serology tests have been developed, but the recent emergence of variants of concern has revealed the need for assays that predict the neutralizing capacity of antibodies in a fast and adaptable manner. Sensitive and fast neutralization assays would allow a timely evaluation of immunity against emerging variants and support drug and vaccine discovery efforts. Here we describe a simple, fast, and cell-free multiplexed flow cytometry assay to interrogate the ability of antibodies to prevent the interaction of Angiotensin-converting enzyme 2 (ACE2) and the receptor binding domain (RBD) of the original Wuhan-1 SARS-CoV-2 strain and emerging variants simultaneously, as a surrogate neutralization assay. Using this method, we demonstrate that serum antibodies collected from representative individuals at different time-points during the pandemic present variable neutralizing activity against emerging variants, such as Omicron BA.1 and South African B.1.351. Importantly, antibodies present in samples collected during 2021, before the third dose of the vaccine was administered, do not confer complete neutralization against Omicron BA.1, as opposed to samples collected in 2022 which show significant neutralizing activity. The proposed approach has a comparable performance to other established surrogate methods such as cell-based assays using pseudotyped lentiviral particles expressing the spike of SARS-CoV-2, as demonstrated by the assessment of the blocking activity of therapeutic antibodies (i.e. Imdevimab) and serum samples. This method offers a scalable, cost effective and adaptable platform for the dynamic evaluation of antibody protection in affected populations against variants of SARS-CoV-2.

Otras publicaciones de la misma revista o congreso con autores/as de la Universidad de Cantabria

 Fuente: Frontiers in immunology, 2022, 13, 1014309

Editorial: Frontiers Research Foundation

 Fecha de publicación: 01/11/2022

Nº de páginas: 10

Tipo de publicación: Artículo de Revista

 DOI: 10.3389/fimmu.2022.1014309

ISSN: 1664-3224

 Proyecto español: BIO21/COV/037

Url de la publicación: https://doi.org/10.3389/fimmu.2022.1014309

Autoría

EGIA-MENDIKUTE, LEIRE

BOSCH, ALEXANDRE

PRIETO-FERNÁNDEZ, ENDIKA

VILA-VECILLA, LAURA

ROMINA ZANETTI, SAMANTA

YOUNG LEE, SO

JIMÉNEZ-LASHERAS, BORJA

GARCÍA DEL RÍO, ANA

ANTOÑANA-VILDOSOLA, ASIER

BLAS, ANDER DE

VELASCO-BELTRÁN, PALOMA

SERRANO-MACIÁ, MARINA

PAULA IRUZUBIETA COZ

MEHRPOUYAN, MAJID

GOLDBERG, EDWUARD M.

BORNHEIMER, SCOTT J.

EMBADE, NIEVES

MARTÍNEZ-CHANTAR, MARÍA L.

MATO, JOSÉ M.