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 Detalle_Publicacion

DNA damage triggers squamous metaplasia in human lung and mammary cells via mitotic checkpoints

Abstract: Epithelial transdifferentiation is frequent in tissue hyperplasia and contributes to disease in various degrees. Squamous metaplasia (SQM) precedes epidermoid lung cancer, an aggressive and frequent malignancy, but it is rare in the epithelium of the mammary gland. The mechanisms leading to SQM in the lung have been very poorly investigated. We have studied this issue on human freshly isolated cells and organoids. Here we show that human lung or mammary cells strikingly undergo SQM with polyploidisation when they are exposed to genotoxic or mitotic drugs, such as Doxorubicin or the cigarette carcinogen DMBA, Nocodazole, Taxol or inhibitors of Aurora-B kinase or Polo-like kinase. To note, the epidermoid response was attenuated when DNA repair was enhanced by Enoxacin or when mitotic checkpoints where abrogated by inhibition of Chk1 and Chk2. The results show that DNA damage has the potential to drive SQM via mitotic checkpoints, thus providing novel molecular candidate targets to tackle lung SCC. Our findings might also explain why SCC is frequent in the lung, but not in the mammary gland and why chemotherapy often causes complicating skin toxicity.

Otras publicaciones de la misma revista o congreso con autores/as de la Universidad de Cantabria

 Fuente: Cell Death Discovery, 2023, 9(1), 21

Editorial: Springer Nature

 Año de publicación: 2023

Nº de páginas: 12

Tipo de publicación: Artículo de Revista

 DOI: 10.1038/s41420-023-01330-3

ISSN: 2058-7716

Autoría

SAN JUAN, LUCÍA

FREIJE, ANA

SANZ-GÓMEZ, NATALIA

JIMÉNEZ-MATÍAS, BEATRIZ

PLEGUEZUELOS-MANZANO, CAYETANO

JUAN RAMON SANZ GIMENEZ-RICO

CLEVERS, HANS

GANDARILLAS, ALBERTO