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One-pot synthesis of compact DNA silica particles for gene delivery and extraordinary DNA preservation

Abstract: Repairing genetic defects using exogenous DNA is a major challenge the science is currently facing. This requires the design of vectors that can effectively encapsulate, protect and target nucleic acids to specific cells safely and precisely. Here we have designed silica-based physiologically responsive particles to encapsulate, store, and transfer DNA. Unlike existing vectors (e.g., viral or lipidic particles), these DNA@SiO2 systems are very stable at room temperature. We also demonstrate how they protect the encapsulated DNA from exposure to different biological and physicochemical stresses, including DNase, denaturation temperatures (>100 °C), or reactive oxygen species (ROS). Remarkably, upon cellular uptake, these vectors dissolve safely unpacking the DNA and transfecting the cells.

 Autoría: Ramos-Valle A., Marín-Caba L., Hevia L.G., Correa-Duarte M.A., Fanarraga M.L.,

 Fuente: Materials Today Advances, 2023, 18, 100357

 Año de publicación: 2023

 Nº de páginas: 12

 Tipo de publicación: Artículo de Revista

 DOI: 10.1016/j.mtadv.2023.100357

 ISSN: 2590-0498

 Url de la publicación: https://doi.org/10.1016/j.mtadv.2023.100357