Estamos realizando la búsqueda. Por favor, espere...
1575
37
170
28821
4394
2595
347
299
Abstract: Background: Based on the excellent results of the clinical trials with ALK-inhibitors, the importance of accurately identifying ALK positive lung cancer has never been greater. However, there are increasing number of recent publications addressing discordances between FISH and IHC. The controversy is further fuelled by the different regulatory approvals. This situation prompted us to investigate two ALK IHC antibodies (using a novel ultrasensitive detection-amplification kit) and an automated ALK FISH scanning system (FDA-cleared) in a series of non-small cell lung cancer tumor samples. Methods: Forty-seven ALK FISH-positive and 56 ALK FISH-negative NSCLC samples were studied. All specimens were screened for ALK expression by two IHC antibodies (clone 5A4 from Novocastra and clone D5F3 from Ventana) and for ALK rearrangement by FISH (Vysis ALK FISH break-apart kit), which was automatically captured and scored by using Bioview's automated scanning system. Results: All positive cases with the IHC antibodies were FISH-positive. There was only one IHC-negative case with both antibodies which showed a FISH-positive result. The overall sensitivity and specificity of the IHC in comparison with FISH were 98% and 100%, respectively. Conclusions: he specificity of these ultrasensitive IHC assays may obviate the need for FISH confirmation in positive IHC cases. However, the likelihood of false negative IHC results strengthens the case for FISH testing, at least in some situations.
Fuente: PLoS One , 2014, 9(9),107200
Editorial: Public Library of Science
Año de publicación: 2014
Nº de páginas: 9
Tipo de publicación: Artículo de Revista
DOI: 10.1371/journal.pone.0107200
ISSN: 1932-6203
Consultar en UCrea Leer publicación
CONDE, ESTHER
SUÁREZ-GUATHIER, ANA
BENITO, AMPARO
GARRIDO, PILAR
GARCÍA-CAMPELO, ROSARIO
BISCUOLA, MICHELE
PAZ-ARES, LUIS
HARDISSON, DAVID
DE CASTRO, JAVIER
CARMEN CAMACHO, M.
RODRIGUEZ ABREU, DELVYS
ADBULKADER, IHAB
RAMIREZ, JOSEP
REGUART, NOEMÍ
SALIDO, MARTA
PIJUÁN, LARA
ARRIOLA, EDURNE
SANZ, JULIÁN
JOSE JAVIER GOMEZ ROMAN
FOLGUERAS, VICTORIA
Volver