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Abstract: The survival of motor neuron (SMN) protein plays an essential role in the biogenesis of spliceosomal snRNPs and the molecular assembly of Cajal bodies (CBs). Deletion of or mutations in the SMN1 gene cause spinal muscular atrophy (SMA) with degeneration and loss of motor neurons. Reduced SMN levels in SMA lead to deficient snRNP biogenesis with consequent splicing pathology. Here, we demonstrate that SMN is a novel and specific target of the acetyltransferase CBP (CREB-binding protein). Furthermore, we identify lysine (K) 119 as the main acetylation site in SMN. Importantly, SMN acetylation enhances its cytoplasmic localization, causes depletion of CBs, and reduces the accumulation of snRNPs in nuclear speckles. In contrast, the acetylation-deficient SMNK119R mutant promotes formation of CBs and a novel category of promyelocytic leukemia (PML) bodies enriched in this protein. Acetylation increases the half-life of SMN protein, reduces its cytoplasmic diffusion rate and modifies its interactome. Hence, SMN acetylation leads to its dysfunction, which explains the ineffectiveness of HDAC (histone deacetylases) inhibitors in SMA therapy despite their potential to increase SMN levels.
Fuente: Cellular and Molecular Life Sciences : CMLS, 2018, 75(3), 527-546
Editorial: Springer
Año de publicación: 2018
Nº de páginas: 20
Tipo de publicación: Artículo de Revista
DOI: 10.1007/s00018-017-2638-2
ISSN: 1420-682X,1420-9071
Leer publicación
LAFARGA, VANESA
OLGA TAPIA MARTINEZ
SHARMA, SAHIL
BENGOECHEA, ROCIO
STOECKLIN, GEORG
MIGUEL ANGEL LAFARGA COSCOJUELA
MARIA TERESA BERCIANO BLANCO
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