Estamos realizando la búsqueda. Por favor, espere...

Negative feedback and transcriptional overshooting in a regulatory network for horizontal gene transfer

Abstract: Horizontal gene transfer (HGT) is a major force driving bacterial evolution. Because of their ability to cross inter-species barriers, bacterial plasmids are essential agents for HGT. This ability, however, poses specific requisites on plasmid physiology, in particular the need to overcome a multilevel selection process with opposing demands. We analyzed the transcriptional network of plasmid R388, one of the most promiscuous plasmids in Proteobacteria. Transcriptional analysis by fluorescence expression profiling and quantitative PCR revealed a regulatory network controlled by six transcriptional repressors. The regulatory network relied on strong promoters, which were tightly repressed in negative feedback loops. Computational simulations and theoretical analysis indicated that this architecture would show a transcriptional burst after plasmid conjugation, linking the magnitude of the feedback gain with the intensity of the transcriptional burst. Experimental analysis showed that transcriptional overshooting occurred when the plasmid invaded a new population of susceptible cells. We propose that transcriptional overshooting allows genome rebooting after horizontal gene transfer, and might have an adaptive role in overcoming the opposing demands of multilevel selection

 Autoría: Raul Fernandez-Lopez,Irene del Campo,Carlos Revilla,Ana Cuevas,Fernando de la Cruz

 Fuente: PLoS Genet. 2014,10(2), 1004171

 Editorial: Public Library of Science

 Año de publicación: 2014

 Nº de páginas: 15

 Tipo de publicación: Artículo de Revista

 DOI: 10.1371/journal.pgen.1004171

 ISSN: 1553-7390,1553-7404

 Url de la publicación: https://doi.org/10.1371/journal.pgen.1004171