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Abstract: Acinetobacter baumannii is a common cause of health care associated infections worldwide. A. pittii is an opportunistic pathogen also frequently isolated from Acinetobacter infections other than those from A. baumannii. Knowledge of Acinetobacter virulence factors and their role in pathogenesis is scarce. Also, there are no detailed published reports on the interactions between A. pittii and human phagocytic cells. Using confocal laser and scanning electron microscopy, immunofluorescence, and live-cell imaging, our study shows that immediately after bacteria-cell contact, neutrophils rapidly and continuously engulf and kill bacteria during at least 4 hours of infection in vitro. After 3 h of infection, neutrophils start to release neutrophil extracellular traps (NETs) against Acinetobacter. DNA in NETs colocalizes well with human histone H3 and with the specific neutrophil elastase. We have observed that human neutrophils use large filopodia as cellular tentacles to sense local environment but also to detect and retain bacteria during phagocytosis. Furthermore, co-cultivation of neutrophils with human differentiated macrophages before infections shows that human neutrophils, but not macrophages, are key immune cells to control Acinetobacter. Although macrophages were largely activated by both bacterial species, they lack the phagocytic activity demonstrated by neutrophils.
Fuente: Sci. Rep. 2017; 7: 4571
Editorial: Nature Publishing Group
Año de publicación: 2017
Nº de páginas: 11
Tipo de publicación: Artículo de Revista
DOI: 10.1038/s41598-017-04870-8
ISSN: 2045-2322
Consultar en UCrea Leer publicación
LÁZARO DÍEZ, MARÍA
ITZIAR CHAPARTEGUI GONZALEZ
SANTIAGO REDONDO SALVO
LEIGH, CHIKE
DAVID MERINO FERNANDEZ
DAVID SAN SEGUNDO ARRIBAS
JESUS NAVAS MENDEZ
JOSE MANUEL ICARDO DE LA ESCALERA
ACOSTA, FÉLIX
ALAIN ANTONIO OCAMPO SOSA
MARTÍNEZ MARTÍNEZ, LUIS
JOSE RAMOS VIVAS
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