Estamos realizando la búsqueda. Por favor, espere...
1558
37
171
26527
4215
2554
339
Abstract: Biogenesis and function of microRNAs can be influenced by genetic variants in the pri-miRNA sequences leading to phenotypic variability. This study aims to identify single nucleotide polymorphisms (SNPs) affecting the expression levels of bone-related mature microRNAs and thus, triggering an osteoporotic phenotype. An association analysis of SNPs located in pri-miRNA sequences with bone mineral density (BMD) was performed in the OSTEOMED2 cohort (n = 2183). Functional studies were performed for assessing the role of BMD-associated miRNAs in bone cells. Two SNPs, rs6430498 in the miR-3679 and rs12512664 in the miR-4274, were significantly associated with femoral neck BMD. Further, we measured these BMD-associated microRNAs in trabecular bone from osteoporotic hip fractures comparing to non-osteoporotic bone by qPCR. Both microRNAs were found overexpressed in fractured bone. Increased matrix mineralization was observed after miR-3679-3p inhibition in human osteoblastic cells. Finally, genotypes of rs6430498 and rs12512664 were correlated with expression levels of miR-3679 and miR-4274, respectively, in osteoblasts. In both cases, the allele that generated higher microRNA expression levels was associated with lower BMD values. In conclusion, two osteoblastexpressed microRNAs, miR-3679 and miR-4274, were associated with BMD; their overexpression could contribute to the osteoporotic phenotype. These findings open new areas for the study of bone disorders.
Fuente: Sci Rep 2017 Mar 31; 7(1): 516
Editorial: Nature Publishing Group
Fecha de publicación: 01/03/2017
Nº de páginas: 10
Tipo de publicación: Artículo de Revista
DOI: 10.1038/s41598-017-00641-7
ISSN: 2045-2322
Url de la publicación: http://dx.doi.org/10.1038/s41598-017-00641-7
Consultar en UCrea Leer publicación
UGARTE, LAURA DE
CARO MOLINA, ENRIQUE
RODRÍGUEZ SANZ, MARIA
GARCÍA PÉREZ, MIGUEL ANGEL
JOSE MANUEL OLMOS MARTINEZ
SOSA HENRÍQUEZ, MANUEL
PÉREZ CANO, RAMÓN
GÓMEZ ALONSO, CARLOS
RIO, LUIS DEL
MATEO AGUDO, JESÚS
BLÁZQUEZ CABRERA, JOSÉ ANTONIO
JESUS MARIA HERMOGENES GONZALEZ MACIAS
PINO MONTES, JAVIER DEL
MUÑOZ TORRES, MANUEL
DIAZ CURIEL, MANUEL
MALOUF, JORGE
CANO, ANTONIO
PÉREZ CASTRILLON, JOSÉ LUIS
NOGUES, XAVIER
GARCIA GIRALT, NATALIA
DIEZ PEREZ, ADOLFO
Volver