Abstract: Fundamental research and drug development for personalized medicine necessitates cell
cultures from defined genetic backgrounds. However, providing sufficient numbers of
authentic cells from individuals poses a challenge. Here, we present a new strategy for rapid
cell expansion that overcomes current limitations. Using a small gene library, we expanded
primary cells from different tissues, donors, and species. Cell-type-specific regimens that
allow the reproducible creation of cell lines were identified. In depth characterization of a
series of endothelial and hepatocytic cell lines confirmed phenotypic stability and functionality.
Applying this technology enables rapid, efficient, and reliable production of unlimited
numbers of personalized cells. As such, these cell systems support mechanistic studies,
epidemiological research, and tailored drug development.
