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Expansion of functional personalized cell systems with specific transgene combinations

Abstract: Fundamental research and drug development for personalized medicine necessitates cell cultures from defined genetic backgrounds. However, providing sufficient numbers of authentic cells from individuals poses a challenge. Here, we present a new strategy for rapid cell expansion that overcomes current limitations. Using a small gene library, we expanded primary cells from different tissues, donors, and species. Cell-type-specific regimens that allow the reproducible creation of cell lines were identified. In depth characterization of a series of endothelial and hepatocytic cell lines confirmed phenotypic stability and functionality. Applying this technology enables rapid, efficient, and reliable production of unlimited numbers of personalized cells. As such, these cell systems support mechanistic studies, epidemiological research, and tailored drug development.

 Fuente: Nature Communications 2018; 9(1):994

 Editorial: Nature Publishing Group

 Año de publicación: 2018

 Nº de páginas: 12

 Tipo de publicación: Artículo de Revista

 DOI: 10.1038/s41467-018-03408-4

 ISSN: 2041-1723

 Url de la publicación: https://dx.doi.org/10.1038/s41467-018-03408-4

Autoría

LIPPS, CHRISTOPH

KLEIN, FRANZISKA

WAHLICHT, TOM

SEIFFERT, VIRGINIA

BUTUEVA, MILADA

ZAUERS, JEANNETTE

TRUSCHEL, THERESA

LUCKNER, MARTIN

KÖSTER, MARIO

MACLEOD, RODERICK

PEZOLDT, JÖRN

HÜHN, JOCHEN

YUAN, QINGGONG

MÜLLER, PETER PAUL

KEMPF, HENNING

ZWEIGERDT, ROBERT

DITTRICH-BREIHOLZ, OLIVER

PUFE, THOMAS

MARIA CAROLINA SAÑUDO CAMPO