Abstract: Objective To determine the optimal observation period (OBP) in adults with a clinical diagnosis of brain death (BD) using
electroencephalography (EEG) or computerized tomography angiography (CTA).
Methods We conducted a retrospective observational analysis of adult patients with a diagnosis of BD from January 2000
to February 2017. The optimal OBP was defined as the minimum time interval from the first complete clinical neurological
examination (CNE) that ensures that neither a second CNE nor any ancillary test (AT) performed after this period would
fail to confirm BD.
Results The study sample included 447 patients. In the supratentorial group, the first AT confirmed BD in 389 cases (98%),
but in 8 (2%) cases the complementary test was incongruent. In this group, 8 of 245 patients in whom the first AT was carried
out within the first 2 h after a complete CNE had a non-confirmatory test of BD versus none of 152 in whom the first AT
was delayed more than 2 h (3.0% vs 0.0%; p = 0.026). In the infratentorial group, we found a higher probability of obtaining
a first non-confirmatory AT of BD (34% vs 2%; p = 0.0001) and an OBP greater than 32.5 h was necessary to confirm a BD
diagnosis.
Conclusions We found important differences in the confirmation of BD diagnosis between primary supratentorial and
infratentorial lesion, and identified an optimal OBP of 2 h in patients with supratentorial lesions. By contrast, in primary
posterior fossa/infratentorial lesions, the determination of an optimal OPB remains less accurate and hence more challenging.
