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Free-labeled nanoclay intracellular uptake tracking by confocal Raman imaging

Abstract: Laponite is a nanoplatform that has been successfully used as a new biomaterial for drug delivery, tissue engineering and bioimaging at the nanoscale. In general, a deep knowledge of the mechanism interaction of the nanomaterial with biological components in a physiological environment is highly desirable for properly characterizing its therapeutic efficacy and toxicology. Up to know, the use of fluorescent dyes labelling both, the nanomaterial and cell components, has been a requirement to characterize the cell uptake and to visualize the entrance of the nanomaterial into the cytosol and the cell nucleus. The used of fluorophores usually perturb the physiological medium and can interfere in the nanomaterial cell interaction. A new Raman imaging methodology to track the uptake and internalization of Laponite nanoparticles into J774 macrophages line cells is presented in this work. The combination of Raman spectroscopy and confocal microscopy provides direct information about the localization of the nanoparticle into the cell, through its unique vibrational fingerprint without labelling or adding dyes, and taking advantage of the fact that Laponite and biological molecules bands can be clearly differentiated.

 Autoría: Iturrioz-Rodríguez N., Martín-Rodríguez R., Renero-Lecuna C., Aguado F., González-Legarreta L., González J., Fanarraga M.L., Perdigón A.C.,

 Fuente: Applied Surface Science, 2021, 537, 147870

 Editorial: Elsevier

 Fecha de publicación: 30/01/2021

 Nº de páginas: 30

 Tipo de publicación: Artículo de Revista

 DOI: 10.1016/j.apsusc.2020.147870

 ISSN: 0169-4332

 Proyecto español: PI16/00496 ; PI19/00349

 Url de la publicación: https://doi.org/10.1016/j.apsusc.2020.147870