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Characterization, antifungal susceptibility and virulence of Candida parapsilosis complex isolates in a tertiary hospital in Cantabria, Northern Spain

Abstract: Introduction The correct identification of the species within the Candida parapsilosis complex has become relevant due to the resistance of Candida metapsilosis to antifungals. We describe the characteristics of the Candida parapsilosis complex isolates, with respect to antifungal resistance and biofilm formation. Methods We perform a descriptive cross-sectional study in 30 strains, collected in a tertiary hospital. All strains, were identified by Vitek2, Vitek-MS? systems and by ITS sequencing. The antifungal susceptibility profile was obtained with Sensititre? panels, while biomass production and metabolic activity were quantified by means of crystal violet and XTT reduction assay, respectively. Results There was a 100% correlation between Vitek-MS? and ITS sequencing. All isolates were susceptible to the nine antifungals tested. The metabolic activity and biomass production tests did not show any difference among the subtypes. Conclusions The Vitek-MS? system provides acceptable identification. We did not find significant differences neither in azole resistance nor in biofilm formation.

Otras publicaciones de la misma revista o congreso con autores/as de la Universidad de Cantabria

 Fuente: Enfermedades Infecciosas y Microbiología Clínica, 2023, 41(2), 99-102

Editorial: Doyma

 Año de publicación: 2023

Nº de páginas: 4

Tipo de publicación: Artículo de Revista

 DOI: 10.1016/j.eimc.2021.08.001

ISSN: 0213-005X,1578-1852

Url de la publicación: https://doi.org/10.1016/j.eimc.2021.08.001

Autoría

RUIZ DE ALEGRÍA PUIG, CARLOS

GARCÍA MERINO, MARÍA DEL SOL

MALET PINTOS-FONSECA, ANA DE

JESUS AGÜERO BALBIN