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Cytotoxicity analysis of oxazine 4-perchlorate fluorescence nerve potential clinical biomarker for guided surgery

Abstract: Biological tissue discrimination is relevant in guided surgery. Nerve identification is critical to avoid potentially severe collateral damage. Fluorescence imaging by oxazine 4-perchlorate (O4P) has been recently proposed. In this work, the cytotoxicity of O4P on U87 human-derived glioma cells has been investigated as a function of concentration and operating room irradiation modes. A custom-built optical irradiation device was employed for controlled optical dosimetry. DNA damage and O4P intracellular localization was also investigated by immunofluorescence and confocal microscopy. The results show that concentration below 100 µM can be considered safe. These results contribute to the assessment of the feasibility of O4P as a nerve biomarker.

 Authorship: Pampín-Suárez S., Arce-Diego J.L., Tapia O., Pérez-Campo F.M., Rodríguez-Rey J.C., Fanjul-Vélez F.,

 Fuente: Biomedical Optics Express, 2022, 13(1), 197-208

 Publisher: The Optical Society

 Publication date: 01/01/2022

 No. of pages: 12

 Publication type: Article

 DOI: 10.1364/BOE.447773

 ISSN: 2156-7085

 Spanish project: PGC2018-101464-B-I00

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