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Abstract: Fast, high-throughput methods for measuring the level and duration of protective immune responses to SARS-CoV-2 are needed to anticipate the risk of breakthrough infections. Here we report the development of two quantitative PCR assays for SARS-CoV-2-specific T cell activation. The assays are rapid, internally normalized and probe-based: qTACT requires RNA extraction and dqTACT avoids sample preparation steps. Both assays rely on the quantification of CXCL10 messenger RNA, a chemokine whose expression is strongly correlated with activation of antigen-specific T cells. On restimulation of whole-blood cells with SARS-CoV-2 viral antigens, viral-specific T cells secrete IFN-?, which stimulates monocytes to produce CXCL10. CXCL10 mRNA can thus serve as a proxy to quantify cellular immunity. Our assays may allow large-scale monitoring of the magnitude and duration of functional T cell immunity to SARS-CoV-2, thus helping to prioritize revaccination strategies in vulnerable populations.
Fuente: Nature Biotechnology 2022 Nov;40(11):1680-1689
Publisher: Springer Nature
Year of publication: 2022
No. of pages: 10
Publication type: Article
DOI: 10.1038/s41587-022-01347-6
ISSN: 1087-0156,1546-1696
Publication Url: https://doi.org/10.1038/s41587-022-01347-6
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SCHWARZ, MEGAN
TORRE, DENIS
LOZANO-OJALVO, DANIEL
TAN, ANTHONY T.
TABAGLIO, TOMMASO
MZOUGHI, SLIM
SÁNCHEZ-TEJUELO, RODRIGO
LE BERT, NINA
MING ER LIM, JOEY
HATEM, SANDRA
TUBALLES, KEVIN
CÁMARA, CARMEN
LÓPEZ-GRANADOS, EDUARDO
PAZ-ARTAL, ESTELA
CORREA-ROCHA, RAFAEL
ORTIZ, ALBERTO
MARCOS LOPEZ HOYOS
PORTOLES, JOSÉ
CERVERA, ISABEL
GONZÁLEZ-PÉREZ, MARÍA
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